AGO3 - argonaute RISC catalytic component 3 Gene

Also Known as EIF2C3

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 192669

About AGO3

Cytogenetic location: 1p34.3 Genomic coordinates (GRCh38): 1:35,930,718-36,072,500 (from NCBI)

This gene has 7 transcripts (splice variants), 277 orthologues and 3 paralogues. Ubiquitous expression in skin (RPKM 1.4), testis (RPKM 1.4) and 25 other tissues.

Summary

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

AGO3 Products (2)

mRNA	Protein	Name
NM_024852.4	NP_079128.2	protein argonaute-3 isoform a
NM_177422.3	NP_803171.1	protein argonaute-3 isoform b

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables RNA endonuclease activity	IMP IMP: Inferred from mutant phenotype	29040713	GOA
enables double-stranded RNA binding	IDA IDA: Inferred from direct assay	19966796	GOA
enables endoribonuclease activity, cleaving miRNA-paired mRNA	IMP IMP: Inferred from mutant phenotype	29040713	GOA
enables miRNA binding	IDA IDA: Inferred from direct assay	15260970	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	19167051	GOA
enables single-stranded RNA binding	IDA IDA: Inferred from direct assay	19966796	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in RISC complex assembly	IDA IDA: Inferred from direct assay	19966796	GOA
involved in RNA secondary structure unwinding	IDA IDA: Inferred from direct assay	19966796	GOA
involved in RNA secondary structure unwinding	IMP IMP: Inferred from mutant phenotype	22795694	GOA
involved in mRNA catabolic process	IDA IDA: Inferred from direct assay	18771919	GOA
involved in miRNA processing	IMP IMP: Inferred from mutant phenotype	22795694	GOA
involved in miRNA-mediated gene silencing by inhibition of translation	IDA IDA: Inferred from direct assay	18771919	GOA
NOT involved in miRNA-mediated gene silencing by mRNA destabilization	IDA IDA: Inferred from direct assay	15260970	GOA
involved in pre-miRNA processing	IDA IDA: Inferred from direct assay	19966796	GOA
involved in regulation of stem cell proliferation	IMP IMP: Inferred from mutant phenotype	23064648	GOA
NOT involved in siRNA-mediated gene silencing by mRNA destabilization	IDA IDA: Inferred from direct assay	15260970	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of RISC complex	IDA IDA: Inferred from direct assay	15260970	GOA
part of RISC-loading complex	IDA IDA: Inferred from direct assay	19966796	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	15260970	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

AGO3 Protein Structure

ArgoL1

PAZ

Piwi

0
200
400
600
800
860 a.a.

Protein Preferred Names	Protein Names
protein argonaute-3	argonaute 3, RISC catalytic component

AGO3 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	AGO3	Q9H9G7	EDC4	Homo sapiens	Q6P2E9	Anti Bait CoIP	23064648
Intra	AGO3	Q9H9G7	WTIP	Homo sapiens	A6NIX2	PLA	28683311
Intra	AGO3	Q9H9G7	WTIP	Homo sapiens	A6NIX2	Anti Tag CoIP	28683311
Intra	AGO3	Q9H9G7	WTIP	Homo sapiens	A6NIX2	Confocal	28683311
Intra	AGO3	Q9H9G7	TNRC6A	Homo sapiens	Q8NDV7	Confocal	28683311
Intra	AGO3	Q9H9G7	TNRC6A	Homo sapiens	Q8NDV7	Anti Bait CoIP	28683311
Intra	AGO3	Q9H9G7	IPO8	Homo sapiens	O15397	Density Sedimentation	19167051
Intra	AGO3	Q9H9G7	IPO8	Homo sapiens	O15397	Pull Down	19167051
Intra	AGO3	Q9H9G7	IPO8	Homo sapiens	O15397	Anti Tag CoIP	19167051

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant AGO3 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P74422	AGO3 Protein, Human (sf9, His)	Q9H9G7 (M1-A860)	≥ 85%, as determined by reducing SDS-PAGE.

AGO3 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P810748	AGO3 Antibody (YA9991)	WB, ICC/IF	Human, Mouse
HY-P83379	AGO3 Antibody (YA3124)	WB	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Cartilage-Hair Hypoplasia	Metaphyseal Chondrodysplasia, Mckusick Type CHH Mckusick Type Metaphyseal Chondrodysplasia Metaphyseal Dysplasia Without Hypotrichosis Cartilage Hair Hypoplasia Like Syndrome Metaphyseal Chondrodysplasia Mckusick Type Chhv Cartilage-Hair Hypoplasia Variant, Skeletal Manifestations Only Cartilage-Hair Hypoplasia-Like Skeletal Dysplasia Without Hypotrichosis Or Immunodeficiency Cartilage-Hair Syndrome Mckusick'S Metaphyseal Chondrodysplasia Syndrome Metaphyseal Chondrodysplasia, Recessive Type Autosomal Recessive Metaphyseal Chondrodysplasia

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS00454	AGO3 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS20689	Ago3 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS27200	Ago3 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Felis catus	AGO3	VGNC	VGNC:107484
Rattus norvegicus	AGO3	RGD	RGD:1309163
Macaca mulatta	AGO3	VGNC	VGNC:99838
Mus musculus	AGO3	MGD	MGI:2446634
Canis familiaris	AGO3	VGNC	VGNC:37708
Others	AGO3	NCBI