AHCY Protein, Human (His-SUMO)

AHCY Protein, Human (His-SUMO) is a key cellular enzyme to catalyze the breakdown of S-adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AHCY Protein, Human is a regulator of biological transmethylation in general.

S-Adenosylhomocysteine hydrolase (AHCY) is a key cellular enzyme to catalyze the breakdown of S-adenosylhomocysteine (AdoHcy) to homocysteine and adenosine, preventing its accumulation and subsequent inhibition of methyl transferases (MTs). In case of AHCY deficiency, intracellular distribution and macromolecular organization of AHCY is impaired, thereby disturbing transmethylation processes possibly in all cellular compartments. AHCY deficiency in humans involves serious biochemical aberrations, retarded psychomotor development, myopathy and myelination disorders, and is potentially lethal in early stages of life. The newest findings link AHCY deficiency to hepatocellular carcinoma^[1][2].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P23526 (S2-Y432)

191  [NCBI]

Full Length of Mature Protein

SDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY

Approximately 63.6 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. Turner MA, et, al. Structure and function of S-adenosylhomocysteine hydrolase. Cell Biochem Biophys. 2000;33(2):101-25.  [Content Brief]

  [2]. Grbeša I, et, al. Mutations in S-adenosylhomocysteine hydrolase (AHCY) affect its nucleocytoplasmic distribution and capability to interact with S-adenosylhomocysteine hydrolase-like 1 protein. Eur J Cell Biol. 2017 Sep;96(6):579-590.  [Content Brief]