ALAS2 Protein, Human (His)
ALAS2 Protein catalyzes PLP-dependent condensation of succinyl-CoA and glycine, producing aminolevulinic acid (ALA) with CoA and CO2 by-products. It significantly contributes to heme formation in erythropoiesis, with catalytic activity reaching 75-85% of isoform 1. ALAS2 Protein, Human (His) is the recombinant human-derived ALAS2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
ALAS2 Protein catalyzes PLP-dependent condensation of succinyl-CoA and glycine, producing aminolevulinic acid (ALA) with CoA and CO2 by-products. It significantly contributes to heme formation in erythropoiesis, with catalytic activity reaching 75-85% of isoform 1. ALAS2 Protein, Human (His) is the recombinant human-derived ALAS2 protein, expressed by E. coli , with N-6*His labeled tag.
The ALAS2 protein serves a critical role in heme biosynthesis by catalyzing the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products, as corroborated by various studies. Notably, ALAS2 significantly contributes to heme production during erythropoiesis, essential for hemoglobin synthesis in red blood cells. This enzymatic activity is a pivotal step in the heme biosynthetic pathway, ensuring the generation of ALA, a precursor molecule crucial for subsequent steps in heme synthesis. The catalytic activity of ALAS2 is reported to be 75-85% of isoform 1 activity, emphasizing its importance in providing the necessary building blocks for hemoglobin production and erythropoiesis.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P22557 (Q50-A587)
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Molecular Construction
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N-term
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6*His
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ALAS2 (Q50-A587)
Accession # P22557 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ALAS2; Aminolevulinate, Delta-, Synthase 2 (Sideroblastic/Hypochromic Anemia); Prev. ASB; 5-Aminolevulinate Synthase 2; ALAS-E; 5-Aminoevulinate Synthase 2; 5-Aminolevulinate Synthase, Erythroid-Specific, Mitochondrial; Delta-ALA Synthetase; 5-Aminolevuli
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AA Sequence
QIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA
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Predicted Molecular Mass
63.5 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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