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  3. Oxidoreductases (EC 1)
  4. ALDH4A1 Protein, Human (sf9)

ALDH4A1 Protein, Human (sf9)

Cat. No.: HY-P76138
COA Handling Instructions

The ALDH4A1 protein is a key enzyme that irreversibly converts delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a key step linking the urea and tricarboxylic acid cycles. ALDH4A1 prefers glutamate γ-semialdehyde but also accepts substrates such as succinate, glutaric acid, and adipic acid semialdehyde. ALDH4A1 Protein, Human (sf9) is the recombinant human-derived ALDH4A1 protein, expressed by Sf9 insect cells , with tag free. The total length of ALDH4A1 Protein, Human (sf9) is 539 a.a., with molecular weight of ~54 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The ALDH4A1 protein is a key enzyme that irreversibly converts delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a key step linking the urea and tricarboxylic acid cycles. ALDH4A1 prefers glutamate γ-semialdehyde but also accepts substrates such as succinate, glutaric acid, and adipic acid semialdehyde. ALDH4A1 Protein, Human (sf9) is the recombinant human-derived ALDH4A1 protein, expressed by Sf9 insect cells , with tag free. The total length of ALDH4A1 Protein, Human (sf9) is 539 a.a., with molecular weight of ~54 KDa.

Background

ALDH4A1, a pivotal enzyme, catalyzes the irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived from either proline or ornithine, into glutamate. This enzymatic transformation serves as a crucial step in the intricate pathway linking the urea and tricarboxylic acid cycles. The preferred substrate for ALDH4A1 is glutamic gamma-semialdehyde, while it also accepts other substrates such as succinic, glutaric, and adipic semialdehydes. The enzymatic activity of ALDH4A1 plays a central role in maintaining the metabolic balance between proline, ornithine, and glutamate, contributing to the efficient utilization of these amino acids within the interconnected cellular pathways.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

Tag Free

Accession

P30038 (K25-Q563)

Gene ID
Synonyms
Delta-1-pyrroline-5-carboxylate dehydrogenase; P5C dehydrogenase; ALDH4; P5CDH
Molecular Weight

Approximately 54 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

ALDH4A1 Protein, Human (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ALDH4A1 Protein, Human (sf9)
Cat. No.:
HY-P76138
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