Aldose-reductase Protein, Human (His)
The Aldose-reductase protein reduces carbonyl-containing compounds to alcohols, including retinals. It detoxifies unsaturated carbonyls like crotonaldehyde and 4-hydroxynonenal, but lacks reductase activity towards glucose. Aldose-reductase Protein, Human (His) is the recombinant human-derived Aldose-reductase protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The Aldose-reductase protein reduces carbonyl-containing compounds to alcohols, including retinals. It detoxifies unsaturated carbonyls like crotonaldehyde and 4-hydroxynonenal, but lacks reductase activity towards glucose. Aldose-reductase Protein, Human (His) is the recombinant human-derived Aldose-reductase protein, expressed by E. coli , with N-6*His labeled tag.
Background
Aldose reductase, a multifunctional enzyme, catalyzes the NADPH-dependent reduction of a diverse range of carbonyl-containing compounds, converting them into their corresponding alcohols. Notably, it exhibits robust enzymatic activity towards various retinal isomers, including all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. The enzyme plays a pivotal role in the detoxification of unsaturated carbonyls derived from the diet and lipids, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal, and their glutathione-conjugates carbonyls (GS-carbonyls). Remarkably, despite its broad substrate specificity, Aldose reductase does not display reductase activity towards glucose. This diverse catalytic repertoire highlights its significance in cellular detoxification processes and underscores its potential therapeutic implications in addressing oxidative stress-related conditions.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
O60218 (M1-Y316)
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Gene ID57016
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Molecular Construction
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N-term
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6*His
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Aldose-reductase (M1-Y316)
Accession # O60218 -
C-term
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Protein Length
Full Length
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Synonyms
AKR1B10; HSI; Prev. AKR1B11; Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase); ARL-1; Aldose Reductase-Like Peptide; Aldose Reductase-Related Protein; Aldose Reductase-Like 1; Small Intestine Reductase; SI Reductase; AKR1B12; HARP; ALDRLn; ARP;
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AA Sequence
MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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