ASPN Protein, Human (His-SUMO)
Based on 1 Customer Validation
ASPN protein acts as a negative regulator in periodontal ligament (PDL) differentiation and mineralization, preventing ossification and maintaining homeostasis of the tooth support system. It inhibits BMP2-induced cell differentiation by blocking the binding of BMP2 to BMPR1B, thereby preventing BMP-dependent activation of SMAD proteins. ASPN Protein, Human (His-SUMO) is the recombinant human-derived ASPN protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
ASPN protein acts as a negative regulator in periodontal ligament (PDL) differentiation and mineralization, preventing ossification and maintaining homeostasis of the tooth support system. It inhibits BMP2-induced cell differentiation by blocking the binding of BMP2 to BMPR1B, thereby preventing BMP-dependent activation of SMAD proteins. ASPN Protein, Human (His-SUMO) is the recombinant human-derived ASPN protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
ASPN protein serves as a negative regulator in the differentiation and mineralization processes of the periodontal ligament (PDL), ensuring the prevention of ossification and maintaining the homeostasis of the tooth-supporting system. Its inhibitory action involves blocking BMP2-induced cytodifferentiation of PDL cells by preventing BMP2 binding to the BMPR1B/BMP type-1B receptor, thereby impeding BMP-dependent activation of SMAD proteins. Beyond its role in the PDL, ASPN is a critical regulator of TGF-beta in articular cartilage, where it plays a pivotal role in cartilage homeostasis and contributes to the pathogenesis of osteoarthritis (OA) by negatively regulating chondrogenesis. Furthermore, ASPN interacts with TGFB1, TGFB2, and TGFB3, inhibiting their binding, and engages in calcium binding, participating in osteoblast-driven collagen biomineralization activity. Its intricate interactions with BMP2, type II collagen, and type I collagen underscore its multifaceted involvement in cellular processes that maintain tissue integrity and regulate bone development.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His;N-SUMO
-
Accession
Q9BXN1 (D33-M380)
-
Molecular Construction
-
N-term
-
6*His-SUMO
-
ASPN (D33-M380)
Accession # Q9BXN1 -
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
ASPN; PLAP1; Asporin; Periodontal Ligament Associated Protein 1; SLRR1C; Periodontal Ligament-Associated Protein 1; PLAP-1; Small Leucine-Rich Protein 1C; Asporin (LRR Class 1); Alternative Protein ASPN; Asporin Proteoglycan; ASPN Protein; FLJ20129; OS3
-
AA Sequence
DMEDTDDDDDDDDDDDDDDEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIARVGVNDFCPTVPKMKKSLYSAISLFNNPVKYWEMQPATFRCVLSRMSVQLGNFGM
-
Predicted Molecular Mass
55.7 kDa
-
Molecular Weight
Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
