ATL3 Protein, Human (His)

ATL3 protein is a GTPase that mediates homotypic fusion of endoplasmic reticulum (ER) membranes by promoting membrane tethering through the formation of trans homomers. ATL3 is critical for ER tubular network biogenesis, contributing to the organization and dynamics of this important cellular structure. ATL3 Protein, Human (His) is the recombinant human-derived ATL3 protein, expressed by E. coli , with N-His labeled tag. The total length of ATL3 Protein, Human (His) is 445 a.a., with molecular weight of ~52.6 kDa.

ATL3 Protein serves as a GTPase that facilitates membrane tethering by forming trans-homooligomers, thereby mediating homotypic fusion of endoplasmic reticulum (ER) membranes. This pivotal protein plays a crucial role in the biogenesis of the tubular network within the endoplasmic reticulum, contributing to the organization and dynamics of this essential cellular structure. Additionally, ATL3 engages in interactions with specific proteins, such as ZFYVE27 and REEP5, suggesting a network of molecular associations that further modulate its functional role in membrane dynamics and ER architecture.

Measured by its ability to catalyze the substrate GTP. The specific activity is 613.47 μM/min/μg, as measured under the described conditions.

Human

E. coli

N-10*His

Q6DD88 (M1-A445)

25923  [NCBI]

MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEANNLAAAASAKDIYYNNMEEVCGGEKPYLSPDILEEKHCEFKQLALDHFKKTKKMGGKDFSFRYQQELEEEIKELYENFCKHNGSKNVFSTFRTPA

Approximately 52.6 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.