ATP5F1A Protein, Human (His)

ATP5F1A is an important component of mitochondrial ATP synthase (complex V) that coordinates ATP production from ADP by utilizing the transmembrane proton gradient. As part of the F-type ATPase, the α and β subunits of ATP5F1A form the catalytic core to achieve ATP hydrolysis. ATP5F1A Protein, Human (His) is the recombinant human-derived ATP5F1A protein, expressed by E. coli , with N-6*His labeled tag.

ATP5F1A, a crucial component of the mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V), plays a pivotal role in ATP production from ADP by harnessing the proton gradient across the membrane generated by the respiratory chain's electron transport complexes. Comprising the catalytic core (F(1)) and the membrane proton channel (F(0)), F-type ATPases, like ATP5F1A, consist of multiple subunits with distinct functions. The alpha and beta subunits form the catalytic core in F(1), where rotation of the central stalk against surrounding alpha(3)beta(3) subunits facilitates ATP hydrolysis. Intriguingly, ATP5F1A binds the bacterial siderophore enterobactin, promoting the accumulation of enterobactin-derived iron ions within mitochondria. Additionally, ATP5F1A interacts with various proteins, such as ATPAF2, HRG, PLG, BLOC1S1, BCL2L1 isoform BCL-X(L), CLN5, PPT1, S100A1, and ABCB7, influencing diverse cellular processes ranging from metabolic efficiency and iron homeostasis to apoptotic regulation. Furthermore, ATP5F1A is a crucial component of the ATP synthase complex, highlighting its central role in cellular energy production. Understanding these intricate interactions sheds light on the multifaceted functions of ATP5F1A in cellular physiology.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P25705-1 (Q44-A553)

498  [NCBI]

Full Length of Isoform-1 Mature Protein

QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA

Approximately 56-59.2 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 0.1% SKL.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.