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  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. B3GAT3 Protein, Human (His)

B3GAT3 Protein, Human (His)

Cat. No.: HY-P7626
Handling Instructions

B3GAT3 Protein, Human (His), a recombinant human B3GAT3 produced in E. coli, has a His tag. B3GAT3, a member of the glucuronyltransferase gene family, exhibits strict acceptor specificity.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

B3GAT3 Protein, Human (His), a recombinant human B3GAT3 produced in E. coli, has a His tag. B3GAT3, a member of the glucuronyltransferase gene family, exhibits strict acceptor specificity[1].

Background

Beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) transfers GlcA from UDP-GlcA to the trisaccharide-serine, Galβ1-3Galβ1-4Xylβ1-O-Ser, to form the glycosaminoglycan (GAG)-protein linkage region, GlcAβ1-3Galβ1-3Galβ1-4Xylβ1-O-Ser, common to various proteoglycans[1].

Species

Human

Source

E. coli

Tag

C-6*His

Accession

O94766 (E72-V335)

Gene ID
Synonyms
rHuB3GAT3, His; B3GAT3; Beta-1,3-Glucuronyltransferase 3;
AA Sequence

EALPTIYVVTPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAEGPTPLVSGLLAASGLLFTHLVVLTPKAQRLREGEPGWVHPRGVEQRNKALDWLRGRGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFEEMRWTRGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPSRPFPVDMAGFAVALPLLLDKPNAQFDSTAPRGHLESSLLSHLVDPKDLEPRAANCTRVLVWHTRTEKPKMKQEEQLQRQGRGSDPAIEVHHHHHH

Molecular Weight

31-34 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filter solution of 20 mM Tris, 150 mM NaCl, 2 mM EDTA, 20% Glycerol, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

B3GAT3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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B3GAT3 Protein, Human (His)
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HY-P7626
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