B3GAT3 Protein, Human (His)
B3GAT3 Protein, Human (His), a recombinant human B3GAT3 produced in E. coli, has a His tag. B3GAT3, a member of the glucuronyltransferase gene family, exhibits strict acceptor specificity.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
B3GAT3 Protein, Human (His), a recombinant human B3GAT3 produced in E. coli, has a His tag. B3GAT3, a member of the glucuronyltransferase gene family, exhibits strict acceptor specificity[1].
Background
Beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) transfers GlcA from UDP-GlcA to the trisaccharide-serine, Galβ1-3Galβ1-4Xylβ1-O-Ser, to form the glycosaminoglycan (GAG)-protein linkage region, GlcAβ1-3Galβ1-3Galβ1-4Xylβ1-O-Ser, common to various proteoglycans[1].
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
O94766-1 (E72-V335)
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Molecular Construction
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N-term
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B3GAT3 (E72-V335)
Accession # O94766-1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
B3GAT3; Glucuronosyltransferase I; Beta-1,3-Glucuronyltransferase 3; GlcUAT-I; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 3; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase; GlcAT-I; Sqv-8-Like Protein; Beta-1,3-Gl
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AA Sequence
EALPTIYVVTPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAEGPTPLVSGLLAASGLLFTHLVVLTPKAQRLREGEPGWVHPRGVEQRNKALDWLRGRGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFEEMRWTRGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPSRPFPVDMAGFAVALPLLLDKPNAQFDSTAPRGHLESSLLSHLVDPKDLEPRAANCTRVLVWHTRTEKPKMKQEEQLQRQGRGSDPAIEV
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Predicted Molecular Mass
30.4 kDa
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Molecular Weight
Approximately 31-34 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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