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  4. B3GNT1 Protein, Human (HEK293, His)

B3GNT1 Protein, Human (HEK293, His)

Cat. No.: HY-P7627
Handling Instructions

B3GNT1 Protein, Human (HEK293, His) is related to severe Walker–Warburg syndrome (WWS).

For research use only. We do not sell to patients.

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Description

B3GNT1 Protein, Human (HEK293, His) is related to severe Walker–Warburg syndrome (WWS)[1].

Background

A truncating mutation in B3GNT1 causes severe Walker–Warburg syndrome[1]. B3gnt1 gene encodes a poly-N-acetyl- lactosamine synthase which can initiate and extend poly-N-acetyllactosamine chains [Gal(b1–4)GlcNAc (b1–3)n] [2].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

O43505 (D43-C415)

Gene ID
Molecular Construction
N-term
B3GNT1 (D43-C415)
Accession # O43505
6*His
C-term
Synonyms
rHuB3GNT1, His; B3GNT1; Beta-1,3-N-Acetylglucosaminyltransferase 1;
AA Sequence

DQYFEFFPPSPRSVDQVKAQLRTALASGGVLDASGDYRVYRGLLKTTMDPNDVILATHASVDNLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLAYALSSHCPDMRARVAMHLVCPSRYEAAVPDPREPGEFALLRSCQEVFDKLARVAQPGINYALGTNVSYPNNLLRNLAREGANYALVIDVDMVPSEGLWRGLREMLDQSNQWGGTALVVPAFEIRRARRMPMNKNELVQLYQVGEVRPFYYGLCTPCQAPTNYSRWVNLPEESLLRPAYVVPWQDPWEPFYVAGGKVPTFDERFRQYGFNRISQACELHVAGFDFEVLNEGFLVHKGFKEALKFHPQKEAENQHNKILYRQFKQELKAKYPNSPRRCHHHHHH

Molecular Weight

45-55 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
References

B3GNT1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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B3GNT1 Protein, Human (HEK293, His)
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HY-P7627
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