B7-1/CD80 Protein, Human (HEK293, mFc)
The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (HEK293, mFc) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293, with C-mFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (HEK293, mFc) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293, with C-mFc labeled tag.
Background
The CD80 Protein plays a pivotal role in the costimulatory signal essential for T-lymphocyte activation, facilitating T-cell proliferation and cytokine production upon binding to CD28. Conversely, when interacting with CTLA-4, CD80 elicits opposite effects, inhibiting T-cell activation. In the context of microbial infection, CD80 also acts as a receptor for adenovirus subgroup B, further exemplifying its multifaceted involvement in immune responses and its recognition of diverse signaling cues. The intricate modulation of T-cell activation by CD80 underscores its significance in orchestrating adaptive immune responses and highlights its versatile functions in different physiological contexts.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-mFc
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Accession
P33681-1 (V35-N242)
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Gene ID941
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Molecular Construction
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N-term
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B7-1 (V35-N242)
Accession # P33681 -
mFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CD80; B-Lymphocyte Activation Antigen B7; Prev. CD28LG1; LAB7; Prev. CD28LG; BB1; T-Lymphocyte Activation Antigen CD80; B7; Activation B7-1 Antigen; Costimulatory Molecule Variant IgV-CD80; B7.1; Costimulatory Factor CD80; B7-1; CD80 Molecule; CD80 Antige
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AA Sequence
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN
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Predicted Molecular Mass
50.9 kDa
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Molecular Weight
Approximately 65-85 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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