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beta-Catenin Protein, Human (His-Strep)

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Beta-Catenin protein is an important component of the canonical Wnt pathway, regulating cellular processes. In the absence of Wnt, it complexes with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B to promote CTNNB1 phosphorylation and degradation. beta-Catenin Protein, Human (His-Strep) is a recombinant beta-Catenin protein expressed by E. coli with an N-His; N-StrepII tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Beta-Catenin protein is an important component of the canonical Wnt pathway, regulating cellular processes. In the absence of Wnt, it complexes with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B to promote CTNNB1 phosphorylation and degradation. beta-Catenin Protein, Human (His-Strep) is a recombinant beta-Catenin protein expressed by E. coli with an N-His; N-StrepII tag.

Background

Beta-Catenin protein functions as a key downstream component in the canonical Wnt signaling pathway, regulating various cellular processes. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B, promoting phosphorylation and ubiquitination of CTNNB1 for subsequent degradation. Upon Wnt ligand binding, CTNNB1 accumulates in the nucleus, acting as a coactivator for transcription factors of the TCF/LEF family to activate Wnt-responsive genes. Beyond its role in Wnt signaling, beta-Catenin is involved in cell adhesion, acting as part of an E-cadherin:catenin adhesion complex. It plays a role in regulating centrosome cohesion, CDK2/PTPN6/CTNNB1/CEACAM1-mediated insulin internalization, and promotes neurogenesis. Additionally, beta-Catenin interacts with various proteins, including AXIN1, SRC, TCF7L2, and others, highlighting its involvement in intricate molecular networks governing diverse cellular functions. The dynamic interactions and dual roles of beta-Catenin underscore its significance in cellular homeostasis and signaling cascades.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His;N-StrepⅡ
  • Accession

    P35222 (M1-L781)

  • Gene ID
    1499
  • Protein Length

    Full Length

  • Synonyms

    CTNNB1; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Prev. CTNNB; CDNA FLJ57878, Highly Similar To Beta-Catenin; Catenin Beta-1; Cadherin-Associated Protein; Beta-Catenin; NEDSDV; Armadillo; MRD19; Catenin (Cadherin-Associated Protein), Beta 1, 8

  • AA Sequence

    MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL

  • Molecular Weight

    Approximately 100 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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