beta-Catenin Protein, Human (His)
Based on 1 Customer Validation
Beta-Catenin protein is an important component of the canonical Wnt pathway and regulates cellular processes. In the absence of Wnt, it complexes with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B to promote CTNNB1 phosphorylation and degradation. beta-Catenin Protein, Human (His) is the recombinant human-derived beta-Catenin protein, expressed by E. coli, with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Beta-Catenin protein is an important component of the canonical Wnt pathway and regulates cellular processes. In the absence of Wnt, it complexes with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B to promote CTNNB1 phosphorylation and degradation. beta-Catenin Protein, Human (His) is the recombinant human-derived beta-Catenin protein, expressed by E. coli, with N-His labeled tag.
Background
Beta-Catenin protein functions as a key downstream component in the canonical Wnt signaling pathway, regulating various cellular processes. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B, promoting phosphorylation and ubiquitination of CTNNB1 for subsequent degradation. Upon Wnt ligand binding, CTNNB1 accumulates in the nucleus, acting as a coactivator for transcription factors of the TCF/LEF family to activate Wnt-responsive genes. Beyond its role in Wnt signaling, beta-Catenin is involved in cell adhesion, acting as part of an E-cadherin:catenin adhesion complex. It plays a role in regulating centrosome cohesion, CDK2/PTPN6/CTNNB1/CEACAM1-mediated insulin internalization, and promotes neurogenesis. Additionally, beta-Catenin interacts with various proteins, including AXIN1, SRC, TCF7L2, and others, highlighting its involvement in intricate molecular networks governing diverse cellular functions. The dynamic interactions and dual roles of beta-Catenin underscore its significance in cellular homeostasis and signaling cascades.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
P35222 (N138-E686)
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Gene ID1499
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Molecular Construction
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N-term
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His
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beta-Catenin (N138-E686)
Accession # P35222 -
C-term
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Protein Length
Partial
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Synonyms
CTNNB1; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Prev. CTNNB; CDNA FLJ57878, Highly Similar To Beta-Catenin; Catenin Beta-1; Cadherin-Associated Protein; Beta-Catenin; NEDSDV; Armadillo; MRD19; Catenin (Cadherin-Associated Protein), Beta 1, 88kDa; EVR7; Catenin Beta 1; Catenin (Cadherin-Associated Protein), Beta 1
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AA Sequence
NLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTE
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Predicted Molecular Mass
62.1 kDa
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Molecular Weight
Approximately 60-65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as 0.2 μm filtered solution of 0.1 M Sodium citrate, 150 mM NaCl, pH 5.5 with 25% glycerol as protectant.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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