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Beta-galactosidase/GLB1 Protein, E.coli

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Beta-galactosidase/GLB1 protein is a hydrolase with catalytic activity and binding sites of Mg2+, Mn2+ and Na+. Beta-galactosidase/GLB1 protein can be used for research in genetics, molecular biology, and other life sciences. Beta-galactosidase/GLB1 Protein, E.coli is the recombinant E. coli-derived Beta-galactosidase/GLB1 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: E.coli
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Beta-galactosidase/GLB1 Protein, E.coli
  • Beta-galactosidase/GLB1 Protein, E.coli Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Beta-galactosidase/GLB1 protein is a hydrolase with catalytic activity and binding sites of Mg2+, Mn2+ and Na+. Beta-galactosidase/GLB1 protein can be used for research in genetics, molecular biology, and other life sciences. Beta-galactosidase/GLB1 Protein, E.coli is the recombinant E. coli-derived Beta-galactosidase/GLB1 protein, expressed by E. coli , with tag free.

Background

Beta-galactosidase is a hydrolase produced by Escherichia coli, which is widely found in natural plants. Beta-galactosidase is a tetrimer of four identical, abnormally long polypeptide chains, consisting of 9-10 subunits, with amino acid composition very different from that of Colithinase. Beta-galactosidase can be combined with Mg2+, Mn2+ and Na+. Beta-galactosidase is inhibited by phenethylthiobeta-d-galactose (PETG), isopropyl thiobeta-d-galactose (IPTG), L-ribose, d-galactolactone, lactose, and 2-amino-d-galactose. Beta-galactosidase has a large molecular weight and is easy to be isolated and purified. Beta-galactosidase can be used in food research[1][2][3][4][5].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species E.coli
  • Source E. coli
  • Tag Tag Free
  • Accession

    P00722 (M1-K1024, M443L, C500S, 12-41a.a deletion)

  • Gene ID
    945006  [NCBI]
  • Molecular Construction
    • N-term
    • Beta-gal (M1-K1024, M443L, C500S, 12-41a.a deletion)
      Accession # P00722
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    JW0335; b0344; lacZ; Lactase; Beta-gal; EC:3.2.1.23; Beta-galactosidase

  • AA Sequence

    MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK

  • Predicted Molecular Mass

    112.9 kDa

  • Molecular Weight

    Approximately 115 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 8% trehalose, 0.05% Tween 80, pH 8.1.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Beta-galactosidase/GLB1 Protein, E.coli Product Image
    1/16
  • Experimental Validation Results for Beta-galactosidase/GLB1 Protein, E.coli
  • Beta-galactosidase/GLB1 Protein, E.coli Product Image