Beta-NGF Protein, Mouse (110a.a, His)

Beta-NGF Protein, vital for developing and maintaining the nervous systems, engages NTRK1 and NGFR receptors, initiating signaling cascades regulating neuronal processes. The NGF precursor, proNGF, acts as a ligand for the SORCS2-NGFR receptor, activating pathways that impact RAC1/2, actin cytoskeleton, and neuronal growth. In vitro, proNGF induces neuronal apoptosis. Beta-NGF inhibits platelet glycoprotein VI proteolysis, binds lysophosphatidylinositol and lysophosphatidylserine, influencing mast cell histamine release. Its homodimer interacts with NTRK1, NGFR, and SORCS2, and proNGF binds to SORT1-NGFR, leading to NGF endocytosis. These interactions highlight Beta-NGF's multifaceted roles in cellular responses. Beta-NGF Protein, Mouse (110a.a, His) is the recombinant mouse-derived Beta-NGF protein, expressed by E. coli, with N-6*His labeled tag. The total length of Beta-NGF Protein, Mouse (110a.a, His) is 110 a.a., with molecular weight of ~12.0 kDa.

Beta-NGF, a pivotal factor in the development and maintenance of the sympathetic and sensory nervous systems, acts as an extracellular ligand for the NTRK1 and NGFR receptors, triggering cellular signaling cascades that regulate neuronal proliferation, differentiation, and survival. The immature NGF precursor (proNGF) serves as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, inducing signaling cascades that result in the inactivation of RAC1 and/or RAC2, along with the reorganization of the actin cytoskeleton and subsequent neuronal growth cone collapse. In contrast to mature NGF, proNGF has been identified to promote neuronal apoptosis in vitro. Additionally, Beta-NGF inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binding to lysophosphatidylinositol and lysophosphatidylserine within its homodimeric structure, Beta-NGF exhibits diverse functions in cellular responses, including promoting histamine release from mast cells when in its lipid-bound form. The homodimeric structure of Beta-NGF interacts with NTRK1, NGFR, and SORCS2, while the NGF precursor (proNGF) binds to a receptor complex formed by SORT1 and NGFR, leading to NGF endocytosis. These intricate interactions underscore the multifaceted roles of Beta-NGF in orchestrating neuronal functions and cellular responses.

The cell proliferation assay using TF-1 human erythroleukemic cells wirh an ED50 value of 0.5-9.2 ng/mL.

Mouse

E. coli

N-6*His

P01139 (M130-R239)

18049

MGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATR

Approximately 12.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.