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  4. BLM Protein, Mouse (His-Myc)

BLM Protein, Mouse (His-Myc)

Cat. No.: HY-P72105
Handling Instructions

BLM is an ATP-dependent DNA helicase that can unwind single- and double-stranded DNA in the 3'-5' direction and actively participates in the DNA replication and repair process. Notably, BLM plays a critical role in 5' end resection during double-strand break repair, recruiting DNA2 to cleave 5'-ssDNA. BLM Protein, Mouse (His-Myc) is the recombinant mouse-derived BLM protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of BLM Protein, Mouse (His-Myc) is 176 a.a., with molecular weight of ~24.7 kDa.

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Description

BLM is an ATP-dependent DNA helicase that can unwind single- and double-stranded DNA in the 3'-5' direction and actively participates in the DNA replication and repair process. Notably, BLM plays a critical role in 5' end resection during double-strand break repair, recruiting DNA2 to cleave 5'-ssDNA. BLM Protein, Mouse (His-Myc) is the recombinant mouse-derived BLM protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of BLM Protein, Mouse (His-Myc) is 176 a.a., with molecular weight of ~24.7 kDa.

Background

BLM, an ATP-dependent DNA helicase, plays a pivotal role in unwinding both single- and double-stranded DNA in a 3'-5' direction. It actively participates in DNA replication and repair processes, contributing to the intricate molecular machinery involved in these essential cellular functions (By similarity). Notably, BLM is a key player in the 5'-end resection of DNA during double-strand break (DSB) repair, where it unwinds DNA and recruits DNA2, which mediates the cleavage of 5'-ssDNA. Additionally, BLM exhibits a negative regulatory role in sister chromatid exchange (SCE) . It demonstrates a capacity to stimulate DNA 4-way junction branch migration and DNA Holliday junction dissolution and displays binding affinity for single-stranded DNA (ssDNA), forked duplex DNA, and DNA Holliday junctions (By similarity). The orchestrated recruitment of BLM to DNA replication forks by the KHDC3-OOEP scaffold, coupled with TRIM25 ubiquitination, underscores its involvement in promoting the restart of stalled replication forks.

Species

Mouse

Source

E. coli

Tag

N-10*His;C-Myc

Accession

O88700 (I684-Q859)

Gene ID

12144  [NCBI]

Molecular Construction
N-term
10*His
BLM (I684-Q859)
Accession # O88700
C-term
Synonyms
BlmBloom syndrome protein homolog; mBLM; EC 3.6.4.12; RecQ helicase homolog
AA Sequence

INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ

Molecular Weight

Approximately 24.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

BLM Protein, Mouse (His-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BLM Protein, Mouse (His-Myc)
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HY-P72105
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