BLM Protein, Mouse (His-Myc)
BLM is an ATP-dependent DNA helicase that can unwind single- and double-stranded DNA in the 3'-5' direction and actively participates in the DNA replication and repair process. Notably, BLM plays a critical role in 5' end resection during double-strand break repair, recruiting DNA2 to cleave 5'-ssDNA. BLM Protein, Mouse (His-Myc) is the recombinant mouse-derived BLM protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
BLM is an ATP-dependent DNA helicase that can unwind single- and double-stranded DNA in the 3'-5' direction and actively participates in the DNA replication and repair process. Notably, BLM plays a critical role in 5' end resection during double-strand break repair, recruiting DNA2 to cleave 5'-ssDNA. BLM Protein, Mouse (His-Myc) is the recombinant mouse-derived BLM protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
Background
BLM, an ATP-dependent DNA helicase, plays a pivotal role in unwinding both single- and double-stranded DNA in a 3'-5' direction. It actively participates in DNA replication and repair processes, contributing to the intricate molecular machinery involved in these essential cellular functions (By similarity). Notably, BLM is a key player in the 5'-end resection of DNA during double-strand break (DSB) repair, where it unwinds DNA and recruits DNA2, which mediates the cleavage of 5'-ssDNA. Additionally, BLM exhibits a negative regulatory role in sister chromatid exchange (SCE) . It demonstrates a capacity to stimulate DNA 4-way junction branch migration and DNA Holliday junction dissolution and displays binding affinity for single-stranded DNA (ssDNA), forked duplex DNA, and DNA Holliday junctions (By similarity). The orchestrated recruitment of BLM to DNA replication forks by the KHDC3-OOEP scaffold, coupled with TRIM25 ubiquitination, underscores its involvement in promoting the restart of stalled replication forks.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-10*His;C-Myc
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Accession
O88700 (I684-Q859)
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Molecular Construction
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N-term
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10*His
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BLM (I684-Q859)
Accession # O88700 -
C-term
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Protein Length
Full Length of Helicase ATP-binding Domain
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Synonyms
BLM; Bloom Syndrome Protein; BLM RecQ Like Helicase; RecQ Protein-Like 3; RECQL3; Bloom Syndrome, RecQ Helicase-Like; RECQ2; Bloom Syndrome; RecQ-Like DNA Helicase BLM; BLM Protein; DNA 3'-5' Helicase BLM; MGRISCE1; BS; RECQL2; Bloom Syndrome RecQ Like He
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AA Sequence
INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ
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Predicted Molecular Mass
24.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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