Blood group RhD polypeptide Protein, Human (Cell-Free, His)

RhD blood group polypeptide proteins emerged as potential components of oligomeric complexes, suggesting that they may be involved in structures within the erythrocyte membrane with potential transport or channel functions. Blood group RhD polypeptide Protein, Human (Cell-Free, His) is the recombinant human-derived Blood group RhD polypeptide protein, expressed by E. coli Cell-free , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli Cell-free
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

RhD blood group polypeptide proteins emerged as potential components of oligomeric complexes, suggesting that they may be involved in structures within the erythrocyte membrane with potential transport or channel functions. Blood group RhD polypeptide Protein, Human (Cell-Free, His) is the recombinant human-derived Blood group RhD polypeptide protein, expressed by E. coli Cell-free , with C-10*His labeled tag.

Background

The Blood group RhD polypeptide protein emerges as a potential component of an oligomeric complex, indicating its likely involvement in a structure that may have a transport or channel function within the erythrocyte membrane. Although the specific details of this complex and its precise role in transport or channel activities are yet to be fully elucidated, the association of Blood group RhD polypeptide with such a complex suggests a potential role in modulating the transport functions crucial for erythrocyte membrane integrity and functionality. The versatile nature of this protein within the erythrocyte membrane underscores its potential impact on diverse cellular processes, making it a subject of interest for further exploration to unravel its contribution to the intricate dynamics of erythrocyte function.

Technical Parameters

  • Species Human
  • Source E. coli Cell-free
  • Tag C-10*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • RHD (S2-F417)
      Accession # Q02161
    • 10*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    RHD; Rhesus Blood Group Antigen CE; Prev. RH; Rhesus Blood Group D Antigen; SLC42A5; Rhesus System D Polypeptide; CD240D; Rh Blood Group, D Anitgen; DIIIc; RhD Blood Group D Antigen; RhPI; Rh Blood Group, D Antigen; RhII; Rh Blood Group CE Antigen; Rh4; R

  • AA Sequence

    SSKYPRSVRRCLPLWALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAAIGLGFLTSSFRRHSWSSVAFNLFMLALGVQWAILLDGFLSQFPSGKVVITLFSIRLATMSALSVLISVDAVLGKVNLAQLVVMVLVEVTALGNLRMVISNIFNTDYHMNMMHIYVFAAYFGLSVAWCLPKPLPEGTEDKDQTATIPSLSAMLGALFLWMFWPSFNSALLRSPIERKNAVFNTYYAVAVSVVTAISGSSLAHPQGKISKTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISVGGAKYLPGCCNRVLGIPHSSIMGYNFSLLGLLGEIIYIVLLVLDTVGAGNGMIGFQVLLSIGELSLAIVIALMSGLLTGLLLNLKIWKAPHEAKYFDDQVFWKFPHLAVGF

  • Predicted Molecular Mass

    47.9 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
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Concentration (final) Concentration (final)
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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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