BPI Protein, Human (HEK293, His)
Based on 1 Customer Validation
BPI Protein, Human (HEK293, His), a member of a genomically conserved lipid-interactive protein family, belongs to a diverse group of polypeptides with antimicrobial activity that are thought to participate in antimicrobial host defence.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
BPI Protein, Human (HEK293, His), a member of a genomically conserved lipid-interactive protein family, belongs to a diverse group of polypeptides with antimicrobial activity that are thought to participate in antimicrobial host defence[1][2][3].
Background
BPI consists of two functionally distinct domains: a potently antibacterial and anti-endotoxin amino-terminal domain (-20 kDa) and a carboxy-terminal portion that imparts opsonic activity to BPI[1].
Bactericidal/permeability-increasing protein has endotoxin-neutralizing activity[2].
BPI exhibits strong anti-microbial activity against GNB and potent endotoxin-neutralizing activity[3].
Verified Bioactivity
Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cell. The ED50 for this effect is 3.082 ng/mL in the present of 2 μg/mL LPS, corresponding to a specific activity is 3.245×105 U/mg.
MCE Validation Data
-
Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-6*His
-
Accession
AAH40955.1 (V32-K487)
-
Molecular Construction
-
N-term
-
BPI (V32-K487)
Accession # AAH40955.1 -
6*His
-
C-term
-
-
Protein Length
Full Length of Bactericidal permeability-increasing protein Chain
-
Synonyms
BPI; CAP 57; Bactericidal Permeability Increasing Protein; Bactericidal/Permeability-Increasing Protein; Bactericidal Permeability-Increasing Protein; Recombinant BPI Holoprotein, RBPI; BPIFD1; RBPI; BPI Fold Containing Family D, Member 1
-
AA Sequence
VNPGVVVRISQKGLDYASQQGTAALQKELKRIKIPDYSDSFKIKHLGKGHYSFYSMDIREFQLPSSQISMVPNVGLKFSISNANIKISGKWKAQKRFLKMSGNFDLSIEGMSISADLKLGSNPTSGKPTITCSSCSSHINSVHVHISKSKVGWLIQLFHKKIESALRNKMNSQVCEKVTNSVSSKLQPYFQTLPVMTKIDSVAGINYGLVAPPATTAETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVYK
-
Predicted Molecular Mass
51.6 kDa
-
Molecular Weight
Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 4 mM HCl.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (264 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. P Elsbach, et al. Role of the bactericidal/permeability-increasing protein in host defence. Curr Opin Immunol. 1998 Feb;10(1):45-9. [Content Brief]
[2]. M N Marra, et al. Bactericidal/permeability-increasing protein has endotoxin-neutralizing activity. J Immunol. 1990 Jan 15;144(2):662-6. [Content Brief]
[3]. Hendrik Schultz, et al. The bactericidal/permeability-increasing protein (BPI) in infection and inflammatory disease. Clin Chim Acta. 2007 Sep;384(1-2):12-23. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
