BTN2A1 Protein, Human (Biotinylated, HEK293, His-Avi)
BTN2A1 is a direct Vγ9 TCR ligand. BTN2A1 consists of extracellular domains (IgV and IgC), a transmembrane (TM) domain, a coiled-coil domain (JM), an intracellular B30.2 domain and a C-terminal tail. BTN2A1 is an immune checkpoint targeting Vγ9Vδ2 T cell cytotoxicity against malignant cells. BTN2A1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived BTN2A1 protein, expressed by HEK293, with C-Avi;C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
BTN2A1 is a direct Vγ9 TCR ligand. BTN2A1 consists of extracellular domains (IgV and IgC), a transmembrane (TM) domain, a coiled-coil domain (JM), an intracellular B30.2 domain and a C-terminal tail. BTN2A1 is an immune checkpoint targeting Vγ9Vδ2 T cell cytotoxicity against malignant cells[1][2]. BTN2A1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived BTN2A1 protein, expressed by HEK293, with C-Avi;C-His labeled tag.
Background
Butyrophilins are a family of transmembrane (TM) proteins of which 8 (BTN1A1, BTN2A1/2A2, BTN3A1/3A2/3A3, MOG, and BTNL2) are located in the major histocompatibility complex (MHC) class I region of human chromosome 6. They are structurally similar to the B7 family, possessing immunoglobulin (Ig)C-IgV extracellular domains, a single-pass TM domain, a juxtamembrane (JTM) domain, and, in most members, an intracellular B30.2 domain. butyrophilin 2A1 (BTN2A1) is required for BTN3A-mediated Vγ9Vδ2 T cell cytotoxicity against cancer cells, and that expression of the BTN2A1/BTN3A1 complex is sufficient to trigger Vγ9Vδ2 TCR activation[1].
Although BTN2A1’s intracellular B30.2 domain shares around 50% sequence identity with BTN3A1’s intracellular B30.2 domain, the BTN2A1 B30.2 domain does not bind to HMBPP[2].
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
Q7KYR7-2 (Q29-A248)
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Gene ID11120
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Molecular Construction
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N-term
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BTN2A1 (Q29-A248)
Accession # Q7KYR7-2 -
His-Avi
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
BTN2A1; BTN2.1; Butyrophilin Subfamily 2 Member A1; Butyrophilin, Subfamily 2, Member A1; BT2.1; BK14H9.1; BTF1; DJ3E1.1
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AA Sequence
QFIVVGPTDPILATVGENTTLRCHLSPEKNAEDMEVRWFRSQFSPAVFVYKGGRERTEEQMEEYRGRTTFVSKDISRGSVALVIHNITAQENGTYRCYFQEGRSYDEAILHLVVAGLGSKPLISMRGHEDGGIRLECISRGWYPKPLTVWRDPYGGVAPALKEVSMPDADGLFMVTTAVIIRDKSVRNMSCSINNTLLGQKKESVIFIPESFMPSVSPCA
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Predicted Molecular Mass
28.1 kDa
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Molecular Weight
Approximately 45-53 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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