2. Recombinant Proteins
  3. Immune Checkpoint Proteins
  4. Butyrophilins
  5. BTNL2
  6. BTNL2 Protein, Mouse (HEK293, His)

BTNL2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P7686
Handling Instructions

BTNL2 Protein, Mouse (HEK293, His) is a recombinant mouse BTNL2 produced in HEK293 cells, with His tag. BTNL2 is a member of butyrophilin-like proteins.

For research use only. We do not sell to patients.

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Description

BTNL2 Protein, Mouse (HEK293, His) is a recombinant mouse BTNL2 produced in HEK293 cells, with His tag. BTNL2 is a member of butyrophilin-like proteins[1].
Background

Butyrophilin-like 2 (BTNL2) is a butyrophilin family member with homology to the B7 costimulatory molecules, polymorphisms of which have been recently associated through genetic analyses to sporadic inclusion body myositis and sarcoidosis[1].
Species

Mouse
Source

HEK293
Tag

C-6*His
Accession

O70355 (D27-S452)
Gene ID

547431  [NCBI]
Molecular Construction
N-term
BTNL2 (D27-S452)
Accession # O70355
6*His
C-term
Synonyms
rMuBTNL2, His; Butyrophilin-like protein 2; BTNL2
AA Sequence

DDFRVVGPNLPILAKVGEDALLTCQLLPKRTTAHMEVRWYRSDPDMPVIMYRDGAEVTGLPMEGYGGRAEWMEDSTEEGSVALKIRQVQPSDDGQYWCRFQEGDYWRETSVLLQVAALGSSPNIHVEGLGEGEVQLVCTSRGWFPEPEVHWEGIWGEKLMSFSENHVPGEDGLFYVEDTLMVRNDSVETISCFIYSHGLRETQEATIALSERLQTELASVSVIGHSQPSPVQVGENIELTCHLSPQTDAQNLEVRWLRSRYYPAVHVYANGTHVAGEQMVEYKGRTSLVTDAIHEGKLTLQIHNARTSDEGQYRCLFGKDGVYQEARVDVQVMAVGSTPRITREVLKDGGMQLRCTSDGWFPRPHVQWRDRDGKTMPSFSEAFQQGSQELFQVETLLLVTNGSMVNVTCSISLPLGQEKTARFPLSHHHHHH

Molecular Weight

55-65 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
References

BTNL2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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