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BUP-1 Protein, Human (His)

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Based on 1 Customer Validation

BUP-1 Protein, Human (His) is a recombinant human β-ureidopropionase produced in HEK293 cells, with His tag. Beta-ureidopropionase catalyzes the last step in the pyrimidine degradation pathway.

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  • Species: Human
  • Source: E. coli
  • 保管条件:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for BUP-1 Protein, Human (His)
  • BUP-1 Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
最寄りの代理店をお探しください Your local distributor: Hycultec GmbHHycultec
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • 参考文献
  • Help & FAQs
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生物活性

製品説明

BUP-1 Protein, Human (His) is a recombinant human β-ureidopropionase produced in HEK293 cells, with His tag. Beta-ureidopropionase catalyzes the last step in the pyrimidine degradation pathway[1].

Background

The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively. UP deficiencies are associated with N-carbamyl-beta-amino aciduria and may lead to abnormalities in neurological activity [1].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • アクセッション番号

    Q9UBR1 (M1-E384)

  • 遺伝子ID
    51733  [NCBI]
  • Molecular Construction
    • N-term
    • BUP-1 (M1-E384)
      Accession # Q9UBR1
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • 別名

    UPB1; Beta‑Ureidopropionase 1; BUP1; N‑Carbamoyl‑Beta‑Alanine Amidohydrolase; Beta‑Ureidopropionase; Beta‑Alanine Synthase; Ureidopropionase, Beta; Uncharacterized Protein DKFZp779O1248; DKFZp779O1248; BUP‑1

  • AA Sequence

    MAGAEWKSLEECLEKHLPLPDLQEVKRVLYGKELRKLDLPREAFEAASREDFELQGYAFEAAEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAAIANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRSRDGLLVAKLDLNLCQQVNDVWNFKMTGRYEMYARELAEAVKSNYSPTIVKE

  • Predicted Molecular Mass

    44.2 kDa

  • 分子量

    Approximately 42 kDa, based on SDS-PAGE under reducing conditions.

  • 純度

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

性状

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

輸送条件

Shipping with dry ice.

参考文献

[1]. Entrez Gene: UPB1 ureidopropionase, beta.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始) 濃度 (開始)
×
体積 (開始) 体積 (開始)
=
濃度 (終了) 濃度 (終了)
×
体積 (終了) 体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • BUP-1 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for BUP-1 Protein, Human (His)
  • BUP-1 Protein, Human (His) Product Image