C-Reactive Protein, Human (HEK293)
Based on 1 Customer Validation
C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (HEK293) is the recombinant human-derived C-Reactive protein, expressed by HEK293 , with tag free.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (HEK293) is the recombinant human-derived C-Reactive protein, expressed by HEK293 , with tag free.
Background
C-Reactive Protein (CRP) exhibits various functions related to host defense. It facilitates agglutination, bacterial capsular swelling, phagocytosis, and complement fixation by binding to phosphorylcholine in a calcium-dependent manner. CRP is also capable of interacting with DNA and histones, and it may scavenge nuclear material that is released from damaged circulating cells.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. When human Fc gamma RIIA（HY-P70711）is immobilized at 2 µg/mL (100 µL/well), can bind Recombinant rhCRP Protein binds with an ED50 of 8.128 μg/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag Tag Free
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Accession
P02741-1 (Q19-P224)
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Gene ID1401
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Molecular Construction
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N-term
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C-Reactive (Q19-P224)
Accession # P02741 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
CRP; C-Reactive Protein, Pentraxin-Related; C-Reactive Protein; Pentraxin 1; PTX1
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AA Sequence
QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP
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Predicted Molecular Mass
23 kDa
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Molecular Weight
Approximately 24 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, pH 8.0, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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