CA125 Protein, Rat (HEK293, His)
The CA125 protein acts by binding to MSLN and plays a crucial role in forming a protective and lubricating barrier on mucosal surfaces. This interaction promotes heterotypic cell adhesion and is suggested to play an important role in peritoneal metastasis of ovarian cancer. CA125 Protein, Rat (HEK293, His) is the recombinant Rat-derived CA125 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Help & FAQs
Biological Activity
Description
The CA125 protein acts by binding to MSLN and plays a crucial role in forming a protective and lubricating barrier on mucosal surfaces. This interaction promotes heterotypic cell adhesion and is suggested to play an important role in peritoneal metastasis of ovarian cancer. CA125 Protein, Rat (HEK293, His) is the recombinant Rat-derived CA125 protein, expressed by HEK293 , with C-His labeled tag.
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-His
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Accession
XP_063122364.1 (N8776-P9448)
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Gene ID315451
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Molecular Construction
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N-term
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MUC16 (N8776-P9448)
Accession # XP_063122364.1 -
His
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C-term
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Protein Length
Partial
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Synonyms
CA125, His; CA125 ovarian cancer antigen; CA125; CA125MUC-16
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Predicted Molecular Mass
77.2 kDa
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Molecular Weight
Approximately 33-45 kDa and 90-130 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE. ≥ 90%, as determined by SEC-MALS.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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