Calumenin Protein, Human (HEK293, His)
Based on 1 Customer Validation
Calumenin Protein, Human (HEK293, His), a recombinant human Calumenin produced in HEK293 cells, has a His tag at the N-terminus. Calumenin protein (CP) is located within the endoplasmic reticulum Ca2+ binding proteins. Calumenin as a suppressor of cell migration and tumor metastasis. Calumenin binds to and stabilizes fibulin-1, leading to inactivation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Calumenin Protein, Human (HEK293, His), a recombinant human Calumenin produced in HEK293 cells, has a His tag at the N-terminus. Calumenin protein (CP) is located within the endoplasmic reticulum Ca2+ binding proteins. Calumenin as a suppressor of cell migration and tumor metastasis. Calumenin binds to and stabilizes fibulin-1, leading to inactivation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling[1][2].
Background
Calumenin, belonging to the CREC protein family, is a multiple EF-hand Ca2+-binding protein, and has been found to have unique C-terminal SR retention signal HDEF. CP can combine with ryanodine receptor to active sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) to regulate the release of intracellular calcium, intake, and storage of calcium in order to maintain homeostasis of calcium cycling. CP can also relieve ERS of myocardial cells and suppress ERS-mediated apoptosis. CP can relieve ERS-initiated apoptosis in viral myocarditis[1].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
O43852 (K20-F315)
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Molecular Construction
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N-term
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Calumenin (K20-F315)
Accession # O43852 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CALU; Multiple EF-Hand Protein; Calumenin; Crocalbin-Like Protein; IEF SSP 9302; Crocalbin
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AA Sequence
KPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF
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Molecular Weight
Approximately 40-55 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.0.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Yu Wang, et al. Calumenin relieves cardiac injury by inhibiting ERS-initiated apoptosis during viral myocarditis. Int J Clin Exp Pathol. 2017 Jul 1;10(7):7277-7284. [Content Brief]
[2]. Pengli Zheng, et al. Calumenin and fibulin-1 on tumor metastasis: Implications for pharmacology. Pharmacol Res. 2015 Sep;99:11-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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