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  3. Hydrolases (EC 3)
  4. CANT1 Protein, Human (HEK293, His)

CANT1 Protein, Human (HEK293, His)

Cat. No.: HY-P74355
Handling Instructions

CANT1, a calcium-dependent nucleotidase, exhibits a pronounced preference for UDP, showcasing its enzymatic activity order as UDP > GDP > UTP > GTP. Notably, its activity towards ADP is minimal, and even lower towards ATP, while it does not hydrolyze AMP and GMP. This substrate specificity has been well-documented in various studies. Beyond its nucleotidase function, CANT1 is implicated in proteoglycan synthesis, underscoring its role in cellular processes related to the production of these essential macromolecules. The distinct substrate preferences and involvement in proteoglycan synthesis highlight the nuanced and specialized functions of CANT1 in cellular metabolism. CANT1 Protein, Human (HEK293, His) is the recombinant human-derived CANT1 protein, expressed by HEK293 , with N-His labeled tag. The total length of CANT1 Protein, Human (HEK293, His) is 322 a.a., with molecular weight of ~40 kDa.

For research use only. We do not sell to patients.

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Description

CANT1, a calcium-dependent nucleotidase, exhibits a pronounced preference for UDP, showcasing its enzymatic activity order as UDP > GDP > UTP > GTP. Notably, its activity towards ADP is minimal, and even lower towards ATP, while it does not hydrolyze AMP and GMP. This substrate specificity has been well-documented in various studies. Beyond its nucleotidase function, CANT1 is implicated in proteoglycan synthesis, underscoring its role in cellular processes related to the production of these essential macromolecules. The distinct substrate preferences and involvement in proteoglycan synthesis highlight the nuanced and specialized functions of CANT1 in cellular metabolism. CANT1 Protein, Human (HEK293, His) is the recombinant human-derived CANT1 protein, expressed by HEK293 , with N-His labeled tag. The total length of CANT1 Protein, Human (HEK293, His) is 322 a.a., with molecular weight of ~40 kDa.

Background

CANT1, a calcium-dependent nucleotidase, exhibits a pronounced preference for UDP, showcasing its enzymatic activity order as UDP > GDP > UTP > GTP. Notably, its activity towards ADP is minimal, and even lower towards ATP, while it does not hydrolyze AMP and GMP. This substrate specificity has been well-documented in various studies. Beyond its nucleotidase function, CANT1 is implicated in proteoglycan synthesis, underscoring its role in cellular processes related to the production of these essential macromolecules. The distinct substrate preferences and involvement in proteoglycan synthesis highlight the nuanced and specialized functions of CANT1 in cellular metabolism.

Species

Human

Source

HEK293

Tag

N-His

Accession

Q8WVQ1 (G80-I401)

Gene ID
Molecular Construction
N-term
His
CANT1 (G80-I401)
Accession # Q8WVQ1
C-term
Synonyms
Soluble calcium-activated nucleotidase 1; SCAN-1; CANT1; SHAPY
Molecular Weight

Approximately 40 kDa

Purity

Greater than 88% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CANT1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CANT1 Protein, Human (HEK293, His)
Cat. No.:
HY-P74355
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