CANT1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
CANT1 is a calcium-dependent nucleotidase that shows a clear preference for UDP, and the order of its enzymatic activity is UDP > GDP > UTP > GTP. It is worth noting that it has little activity on ADP and even less activity on ATP, but it does not hydrolyze AMP and GMP. CANT1 Protein, Human (HEK293, His) is the recombinant human-derived CANT1 protein, expressed by HEK293 , with N-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CANT1 is a calcium-dependent nucleotidase that shows a clear preference for UDP, and the order of its enzymatic activity is UDP > GDP > UTP > GTP. It is worth noting that it has little activity on ADP and even less activity on ATP, but it does not hydrolyze AMP and GMP. CANT1 Protein, Human (HEK293, His) is the recombinant human-derived CANT1 protein, expressed by HEK293 , with N-His labeled tag.
Background
CANT1, a calcium-dependent nucleotidase, exhibits a strong preference for UDP as its substrate, with the order of activity being UDP > GDP > UTP > GTP. Notably, it demonstrates very low activity towards ADP and even lower activity towards ATP, while it does not hydrolyze AMP and GMP. This enzymatic profile suggests a specific role in nucleotide metabolism. CANT1 is implicated in proteoglycan synthesis, indicating its involvement in processes related to the modification and regulation of these important macromolecules. The calcium-dependent nature of CANT1 highlights its regulation by intracellular calcium levels, adding an additional layer of complexity to its enzymatic function and potential cellular roles.
Verified Bioactivity
Measured by its ability to hydrolyze the beta 5'-phosphate group from the substrate uridine-5'-diphosphate (UDP) that incubate at room temperature for 20 min at 37°C. The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is 285089.234 pmol/min/μg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag N-6*His
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Accession
Q8WVQ1-1 (G80-I401)
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Molecular Construction
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N-term
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His
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CANT1 (G80-I401)
Accession # Q8WVQ1-1 -
C-term
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Protein Length
Partial
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Synonyms
CANT1; Apyrase Homolog; Calcium Activated Nucleotidase 1; Micromelic Dwarfism With Vertebral And Metaphyseal Abnormalities And Advanced Carpotarsal Ossification; SCAN-1; Ca2+-Dependent Endoplasmic Reticulum Nucleoside Diphosphatase; SHAPY; Soluble Calcium
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AA Sequence
GQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI
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Molecular Weight
Approximately 40 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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