1. Recombinant Proteins
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  3. Capsid protein, Hepatitis B Virus (His)

Capsid protein, Hepatitis B Virus (His)

Cat. No.: HY-P74354
COA Handling Instructions

Capsid proteins play a key role in the assembly of icosahedral capsids, forming T=4 and T=3 particles. Transported along microtubules to the nucleus, its phosphorylation exposes a nuclear localization signal that promotes binding to nuclear pore complexes via α and β import. Capsid protein, Hepatitis B Virus (His) is the recombinant Virus-derived Capsid protein, expressed by E. coli , with C-His labeled tag. The total length of Capsid protein, Hepatitis B Virus (His) is 149 a.a., with molecular weight of ~17.67 kDa.

For research use only. We do not sell to patients.

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Description

Capsid proteins play a key role in the assembly of icosahedral capsids, forming T=4 and T=3 particles. Transported along microtubules to the nucleus, its phosphorylation exposes a nuclear localization signal that promotes binding to nuclear pore complexes via α and β import. Capsid protein, Hepatitis B Virus (His) is the recombinant Virus-derived Capsid protein, expressed by E. coli , with C-His labeled tag. The total length of Capsid protein, Hepatitis B Virus (His) is 149 a.a., with molecular weight of ~17.67 kDa.

Background

The Capsid Protein plays a crucial role in the assembly of icosahedral capsids, forming large particles with T=4 icosahedral symmetry composed of 240 capsid protein copies, while a fraction assembles into smaller T=3 particles with 180 capsid proteins. These capsids are transported along microtubules to the nucleus, where phosphorylation of the capsid induces the exposure of a nuclear localization signal in its C-terminal portion, facilitating binding to the nuclear pore complex via importin (karyopherin-) alpha and beta. The intact capsids are imported through the nuclear pore into the nuclear basket, likely binding to NUP153. Only mature capsids containing the viral genome can release viral DNA and capsid protein into the nucleoplasm, while immature capsids remain stuck in the basket. Capsids encapsulate pre-genomic RNA and the P protein, with the RNA reverse-transcribed into DNA in the cytoplasm. The capsid, post-reverse transcription, can be directed to the nucleus for additional transcription or bud through the endoplasmic reticulum to produce new virions. The Capsid Protein homodimerizes, then multimerizes, and interacts with cytosol-exposed regions of viral L glycoprotein in the reticulum-to-Golgi compartment. Moreover, it interacts with human FLNB and, in its phosphorylated form, interacts with host importin alpha, dependent on NLS exposure influenced by genome maturation and/or capsid protein phosphorylation. The Capsid Protein also interacts with host NUP153 in the intricate process of viral assembly and genome release.

Species

Virus

Source

E. coli

Tag

C-His

Accession

P03146-1 (M1-V149)

Gene ID

944568  [NCBI]

Molecular Construction
N-term
Capsid (M1-V149)
Accession # P03146-1
His
C-term
Synonyms
Capsid protein; Core antigen; C protein; HBcAg
Molecular Weight

Approximately 17.67 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 150 mM NaCl, 50 mM Tris, 0.5 mM EDTA, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Capsid protein, Hepatitis B Virus (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Capsid protein, Hepatitis B Virus (His)
Cat. No.:
HY-P74354
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