Caspase-1/CASP1 Protein, Human (His)

Measured by its ability convert the substrate acetyl-Tyr-Val-Ala-Asp p-nitroanilide (Ac-YVAD-pNA) to p-nitroaniline (pNA). The specific activity is >50 pmol/min/µg.

Human

E. coli

N-His

P29466-1 (N120-D297&A317-H404)

834

Approximately 9 kDa & 23-25 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 0.5 M Arginine, pH8.0 with 20% glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.