CCDC47 Protein, Mouse (HEK293, His)

CCDC47 protein is a crucial component of the multi-pass translocon (MPT) complex responsible for mediating the insertion of multi-pass membrane proteins into the lipid bilayer of membranes. Functioning in conjunction with the SEC61 complex, the MPT complex takes charge after the initial membrane insertion by the SEC61 complex, occluding the lateral gate of SEC61 to facilitate the insertion of subsequent transmembrane regions. Within the MPT complex, CCDC47, as part of the PAT subcomplex, plays a vital role in sequestering highly polar regions in transmembrane domains away from the non-polar membrane environment until they can be buried within the fully assembled protein. Notably, CCDC47 is implicated in the regulation of calcium ion homeostasis in the endoplasmic reticulum (ER) and is essential for proper protein degradation through the ER-associated degradation (ERAD) pathway. Furthermore, CCDC47 is indispensable for maintaining ER organization during embryogenesis. As a component of the PAT complex, which includes WDR83OS/Asterix, CCDC47 collaborates with other subcomplexes within the MPT complex, such as the GEL complex and the BOS complex, highlighting its integral role in the intricate processes of membrane protein insertion and ER homeostasis. Additionally, CCDC47 interacts with various proteins, including VCP, HSPA5, DERL1, DERL2, and SELENOS, contributing to its diverse functional repertoire.