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CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi)

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CCN2/CTGF (Connective Tissue Growth Factor) is a protein produced primarily by endothelial cells in blood vessels. It plays an important role in various cellular processes. CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CCN2/CTGF protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

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  • Species: Human
  • Source: HEK293
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
  • CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • Help & FAQs
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生物活性

製品説明

CCN2/CTGF (Connective Tissue Growth Factor) is a protein produced primarily by endothelial cells in blood vessels. It plays an important role in various cellular processes. CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CCN2/CTGF protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

CCN2/CTGF (Connective Tissue Growth Factor) is a protein primarily produced by vascular endothelial cells. It plays a significant role in various cellular processes. One of its main functions is to attract and stimulate the proliferation and differentiation of chondrocytes, which are cells involved in the formation of cartilage. Additionally, CCN2/CTGF mediates cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. This adhesion is dependent on the presence of heparin (a polysaccharide) and divalent cations (such as calcium or magnesium). Furthermore, CCN2/CTGF enhances the DNA synthesis induced by fibroblast growth factors, which are proteins involved in cell growth and repair. Overall, CCN2/CTGF is an important protein that regulates cellular processes such as chondrocyte function, cell adhesion, and DNA synthesis, contributing to the development and maintenance of connective tissues.

Verified Bioactivity

1.Immobilized Anti-CTGF Antibody at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 33.4 ng/ml determined by ELISA.
2.Immobilized Biotinylated Human CTGF, His-Avi Tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 4-8 ng/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-8*His
  • アクセッション番号

    Q5M8T4 (Q27-A349)

  • 遺伝子ID
    1490  [NCBI]
  • Molecular Construction
    • N-term
    • CTGF (Q27-A349)
      Accession # Q5M8T4
    • 8*His-Avi
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • 別名

    IGFBP8; IBP-8; CCN2; NOV2; HCS24; CTGF; CTGRP; Fisp12; MGC102839

  • AA Sequence

    QNCSGPCRCPDEPAPRCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCIFGGTVYRSGESFQSSCKYQCTCLDGAVGCMPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDQTVVGPALAAYRLEDTFGPDPTMIRANCLVQTTEWSACSKTCGMGISTRVTNDNASCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKISKPIKFELSGCTSMKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEVMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

  • Predicted Molecular Mass

    38.4 kDa

  • 分子量

    Approximately 43-53 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • 純度

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

性状

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 200mM L-arginine, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)
=
Mass (in vial)
÷
Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始)
×
体積 (開始)
=
濃度 (終了)
×
体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL

CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) 関連分類

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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  • CCN2/CTGF Protein, Human (Biotinylated, HEK293, His-Avi) Product Image