CD161 Protein, Cynomolgus (HEK293, His)
Based on 1 Customer Validation
CD161 Protein plays a crucial role in inhibiting NK cell cytotoxicity by activating specific acid sphingomyelinase/SMPD1, elevating ceramide levels. Activation stimulates AKT1/PKB and RPS6KA1/RSK1 kinases, enhancing anti-CD3-induced T-cell proliferation. As a lectin, CD161 binds to Gal-alpha(1,3)Gal and N-acetyllactosamine epitopes, acting as a ligand for CLEC2D/LLT1. Existing as a homodimer, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted immune regulatory functions. CD161 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD161 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
CD161 Protein plays a crucial role in inhibiting NK cell cytotoxicity by activating specific acid sphingomyelinase/SMPD1, elevating ceramide levels. Activation stimulates AKT1/PKB and RPS6KA1/RSK1 kinases, enhancing anti-CD3-induced T-cell proliferation. As a lectin, CD161 binds to Gal-alpha(1,3)Gal and N-acetyllactosamine epitopes, acting as a ligand for CLEC2D/LLT1. Existing as a homodimer, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted immune regulatory functions. CD161 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD161 protein, expressed by HEK293 , with C-His labeled tag.
Background
CD161 assumes a crucial role in inhibiting natural killer (NK) cell cytotoxicity. Upon activation, CD161 stimulates specific acid sphingomyelinase/SMPD1, resulting in a significant increase in intracellular ceramide levels. The activation process also leads to the stimulation of AKT1/PKB and RPS6KA1/RSK1 kinases, along with a marked enhancement of T-cell proliferation induced by anti-CD3. Functioning as a lectin, CD161 binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and the N-acetyllactosamine epitope. Furthermore, it acts as a ligand for CLEC2D/LLT1, inhibiting NK cell-mediated cytotoxicity and interferon-gamma secretion in target cells. Existing as a homodimer with disulfide linkage, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted regulatory functions in immune responses[1][2][3].
Verified Bioactivity
Immobilized Cynomolgus CD161, His Tag at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 17 ng/mL determined by ELISA.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-8*His
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Accession
XP_005570142 (Q67-L227)
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Molecular Construction
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N-term
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CD161 (Q67-L227)
Accession # XP_005570142 -
8*His
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C-term
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Protein Length
Partial
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Synonyms
KLRB1; CD161; Prev. NKR; Killer Cell Lectin-Like Receptor Subfamily B Member 1; NKR-P1A; C-Type Lectin Domain Family 5 Member B; CLEC5B; NKRP1A; Natural Killer Cell Surface Protein P1A; CD161 Antigen; HNKR-P1A; HNKR-P1a; NKR-P1; Killer Cell Lectin Like Re
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AA Sequence
QKPSIGKCSVDIQQNRTKTTERPDLLNCPIYWQQVQEKCLLFSHTVNPWNNSLADCSTKESSLLLIQDKDELTRTQNLIHDKAISFWIGLNFSLSEKNWKWINGSFLSSNDLKITGDAKENSCVYISQTSVYSEYCSTEMKWICQKELTLVRNKVSPDSWL
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Predicted Molecular Mass
20.2 kDa
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Molecular Weight
Approximately 38-48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5-8% trehalose is added as protectant before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Pozo D, et al. CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase. J Immunol. 2006 Feb 15;176(4):2397-406. [Content Brief]
[2]. Christiansen D, et al. Recognition of a carbohydrate xenoepitope by human NKRP1A (CD161). Xenotransplantation. 2006 Sep;13(5):440-6. [Content Brief]
[3]. Lanier LL, et al. Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J Immunol. 1994 Sep 15;153(6):2417-28. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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