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CD162/PSGL-1 Protein, Mouse (HEK293)

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The CD162/PSGL-1 protein is an SLe(x)-type proteoglycan that mediates rapid rolling of leukocytes through high-affinity interactions with E- and P-selectin, thereby promoting early inflammatory stages.CD162 forms homodimers linked by disulfide bonds and interacts with selectins through the lectin/EGF domain.CD162/PSGL-1 Protein, Mouse (HEK293) is the recombinant mouse-derived CD162/PSGL-1 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • CD162/PSGL-1 Protein, Mouse (HEK293) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The CD162/PSGL-1 protein is an SLe(x)-type proteoglycan that mediates rapid rolling of leukocytes through high-affinity interactions with E- and P-selectin, thereby promoting early inflammatory stages.CD162 forms homodimers linked by disulfide bonds and interacts with selectins through the lectin/EGF domain.CD162/PSGL-1 Protein, Mouse (HEK293) is the recombinant mouse-derived CD162/PSGL-1 protein, expressed by HEK293 , with tag free.

Background

CD162, also known as P-selectin glycoprotein ligand-1 (PSGL-1), functions as an SLe(x)-type proteoglycan and plays a crucial role in the early stages of inflammation by mediating rapid leukocyte rolling over vascular surfaces through high-affinity, calcium-dependent interactions with E- and P-selectins. This interaction is pivotal for the initial capture of leukocytes. CD162 forms homodimers linked by disulfide bonds and interacts with P- and E-selectins through their lectin/EGF domains. For high-affinity binding to P-selectin, sialyl Lewis X glycan modification and tyrosine sulfation, likely on Tyr-54, are required. Dimerization appears dispensable for P-selectin binding. Additionally, CD162 interacts with sorting nexin 20 (SNX20) and mediates SYK activation downstream of P-selectin/SELP binding. Furthermore, CD162 interacts with moesin (MSN) and spleen tyrosine kinase (SYK), contributing to downstream signaling events. It also engages with HAVCR1, revealing its involvement in diverse cellular interactions.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag Tag Free
  • Accession

    Q62170 (L18-C307)

  • Gene ID
    20345  [NCBI]
  • Molecular Construction
    • N-term
    • PSGL-1 (L18-C307)
      Accession # Q62170
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    SELPLG; Cutaneous Lymphocyte-Associated Associated Antigen; Selectin P Ligand; CD162 Antigen; PSGL-1; PSGL1; CD162; CLA; P-Selectin Glycoprotein Ligand 1

  • AA Sequence

    LQLQDPWGHETKEAPGPVHLRERRQVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEAETSQPAPMEADTSQPAPMEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSDLIPVKQC

  • Predicted Molecular Mass

    31.2 kDa

  • Molecular Weight

    Approximately 55 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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