CD162/PSGL-1 Protein, Mouse (HEK293)

The CD162/PSGL-1 protein is an SLe(x)-type proteoglycan that mediates rapid rolling of leukocytes through high-affinity interactions with E- and P-selectin, thereby promoting early inflammatory stages.CD162 forms homodimers linked by disulfide bonds and interacts with selectins through the lectin/EGF domain.CD162/PSGL-1 Protein, Mouse (HEK293) is the recombinant mouse-derived CD162/PSGL-1 protein, expressed by HEK293 , with tag free.

CD162, also known as P-selectin glycoprotein ligand-1 (PSGL-1), functions as an SLe(x)-type proteoglycan and plays a crucial role in the early stages of inflammation by mediating rapid leukocyte rolling over vascular surfaces through high-affinity, calcium-dependent interactions with E- and P-selectins. This interaction is pivotal for the initial capture of leukocytes. CD162 forms homodimers linked by disulfide bonds and interacts with P- and E-selectins through their lectin/EGF domains. For high-affinity binding to P-selectin, sialyl Lewis X glycan modification and tyrosine sulfation, likely on Tyr-54, are required. Dimerization appears dispensable for P-selectin binding. Additionally, CD162 interacts with sorting nexin 20 (SNX20) and mediates SYK activation downstream of P-selectin/SELP binding. Furthermore, CD162 interacts with moesin (MSN) and spleen tyrosine kinase (SYK), contributing to downstream signaling events. It also engages with HAVCR1, revealing its involvement in diverse cellular interactions.

Mouse

HEK293

Tag Free

Q62170 (L18-C307)

20345  [NCBI]

Extracellular Domain

LQLQDPWGHETKEAPGPVHLRERRQVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEAETSQPAPMEADTSQPAPMEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSDLIPVKQC

Approximately 55 kDa, based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.