CD163 Protein, Human (HEK293, His)
The CD163 protein is an acute-phase regulatory receptor that clears hemoglobin/haptoglobin complexes through endocytosis by macrophages, protecting tissues from potential oxidative damage. In addition to iron uptake, CD163 also exhibits calcium- and pH-dependent binding preferences, with higher affinity for the hemoglobin/haptoglobin complex of the HP*1F phenotype. CD163, Human (HEK293, His) is the recombinant human-derived CD163 protein, expressed by HEK293, with labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The CD163 protein is an acute-phase regulatory receptor that clears hemoglobin/haptoglobin complexes through endocytosis by macrophages, protecting tissues from potential oxidative damage. In addition to iron uptake, CD163 also exhibits calcium- and pH-dependent binding preferences, with higher affinity for the hemoglobin/haptoglobin complex of the HP*1F phenotype. CD163, Human (HEK293, His) is the recombinant human-derived CD163 protein, expressed by HEK293, with labeled tag.
Background
CD163 is an acute phase-regulated receptor crucially involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, thereby safeguarding tissues from potential oxidative damage caused by free hemoglobin. Beyond its role in iron uptake and recycling via endocytosis of hemoglobin/haptoglobin, CD163 binds to these complexes in a calcium-dependent and pH-dependent manner. Notably, it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype compared to those of hemoglobin and dimeric haptoglobin of HP*1S phenotype. CD163 induces a cascade of intracellular signals involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and the secretion of IL6 and CSF1. Isoform 3, in particular, demonstrates enhanced ligand endocytosis and surface expression when expressed in cells. The shedding of CD163 produces a soluble form (sCD163) with potential anti-inflammatory properties, serving as a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q86VB7-1 (S42-S1045)
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Gene ID9332
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Molecular Construction
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N-term
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CD163 (S42-S1045)
Accession # Q86VB7-1 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; Soluble CD163; sCD163; CD163; M130
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AA Sequence
SSLGGTDKELRLVDGENKCSGRVEVKVQEEWGTVCNNGWSMEAVSVICNQLGCPTAIKAPGWANSSAGSGRIWMDHVSCRGNESALWDCKHDGWGKHSNCTHQQDAGVTCSDGSNLEMRLTRGGNMCSGRIEIKFQGRWGTVCDDNFNIDHASVICRQLECGSAVSFSGSSNFGEGSGPIWFDDLICNGNESALWNCKHQGWGKHNCDHAEDAGVICSKGADLSLRLVDGVTECSGRLEVRFQGEWGTICDDGWDSYDAAVACKQLGCPTAVTAIGRVNASKGFGHIWLDSVSCQGHEPAIWQCKHHEWGKHYCNHNEDAGVTCSDGSDLELRLRGGGSRCAGTVEVEIQRLLGKVCDRGWGLKEADVVCRQLGCGSALKTSYQVYSKIQATNTWLFLSSCNGNETSLWDCKNWQWGGLTCDHYEEAKITCSAHREPRLVGGDIPCSGRVEVKHGDTWGSICDSDFSLEAASVLCRELQCGTVVSILGGAHFGEGNGQIWAEEFQCEGHESHLSLCPVAPRPEGTCSHSRDVGVVCSRYTEIRLVNGKTPCEGRVELKTLGAWGSLCNSHWDIEDAHVLCQQLKCGVALSTPGGARFGKGNGQIWRHMFHCTGTEQHMGDCPVTALGASLCPSEQVASVICSGNQSQTLSSCNSSSLGPTRPTIPEESAVACIESGQLRLVNGGGRCAGRVEIYHEGSWGTICDDSWDLSDAHVVCRQLGCGEAINATGSAHFGEGTGPIWLDEMKCNGKESRIWQCHSHGWGQQNCRHKEDAGVICSEFMSLRLTSEASREACAGRLEVFYNGAWGTVGKSSMSETTVGVVCRQLGCADKGKINPASLDKAMSIPMWVDNVQCPKGPDTLWQCPSSPWEKRLASPSEETWITCDNKIRLQEGPTSCSGRVEIWHGGSWGTVCDDSWDLDDAQVVCQQLGCGPALKAFKEAEFGQGTGPIWLNEVKCKGNESSLWDCPARRWGHSECGHKEDAAVNCTDISVQKTPQKATTGRS
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Predicted Molecular Mass
110.4 kDa
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Molecular Weight
Approximately 100-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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