CD2 Protein, Cynomolgus/Rhesus Macaque (HEK293, His)

CD2 Protein mediates T-cell adhesion to other cell types by interacting with CD58 (LFA-3) and CD48/BCM1. Implicated in T-cell triggering, its cytoplasmic domain has signaling functions. CD2 also interacts with CD2AP and PSTPIP1. Moreover, its interaction with FCGR3A modulates NK cell activation and cytotoxicity. CD2 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is the recombinant cynomolgus-derived CD2 protein, expressed by HEK293 , with C-6*His labeled tag.

CD2 is a cell surface glycoprotein that plays a crucial role in mediating adhesion between T-cells and various cell types through interactions with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1. Beyond its adhesive function, CD2 is implicated in triggering T-cell responses, with its cytoplasmic domain playing a role in signaling pathways. The protein interacts with CD48, CD58 (LFA-3), CD2AP, and PSTPIP1, contributing to a complex network of molecular interactions. Additionally, the interaction between CD2 and FCGR3A modulates NK cell activation and cytotoxicity, highlighting its diverse involvement in immune cell functions.

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.571 ng/mL, corresponding to a specific activity is 1.7513×10⁶ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.571 ng/mL, corresponding to a specific activity is 1.7513×10⁶ units/mg.

Rhesus Macaque; Cynomolgus

HEK293

C-6*His

Q6SZ61/NP_001182402.1 (K25-D209)

102120798  [NCBI]/677855

Extracellular Domain

KEIRNALETWGALGQDIDLDIPSFQMSDDIDDIRWEKTSDKKKIAQFRKEKETFEEKDAYKLFKNGTLKIKHLKIHDQDSYKVSIYDTKGKNVLEKTFDLKIQERVSEPKISWTCINTTLTCEVMNGTDPELNLYQDGKHVKLSQRVITHKWTTSLSAKFKCTAGNKVSKESRMETVSCPEKGLD

Approximately 33.91 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.