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  5. CD2 Protein, Cynomolgus (HEK293, C-His)

CD2 Protein, Cynomolgus (HEK293, C-His)

Cat. No.: HY-P75434A
COA Handling Instructions

CD2 Protein mediates T-cell adhesion to other cell types by interacting with CD58 (LFA-3) and CD48/BCM1. Implicated in T-cell triggering, its cytoplasmic domain has signaling functions. CD2 also interacts with CD2AP and PSTPIP1. Moreover, its interaction with FCGR3A modulates NK cell activation and cytotoxicity. CD2 Protein, Cynomolgus (HEK293, C-His) is the recombinant cynomolgus-derived CD2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD2 Protein, Cynomolgus (HEK293, C-His) is 185 a.a., with molecular weight of ~33.91 kDa.

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Description

CD2 Protein mediates T-cell adhesion to other cell types by interacting with CD58 (LFA-3) and CD48/BCM1. Implicated in T-cell triggering, its cytoplasmic domain has signaling functions. CD2 also interacts with CD2AP and PSTPIP1. Moreover, its interaction with FCGR3A modulates NK cell activation and cytotoxicity. CD2 Protein, Cynomolgus (HEK293, C-His) is the recombinant cynomolgus-derived CD2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD2 Protein, Cynomolgus (HEK293, C-His) is 185 a.a., with molecular weight of ~33.91 kDa.

Background

CD2 is a cell surface glycoprotein that plays a crucial role in mediating adhesion between T-cells and various cell types through interactions with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1. Beyond its adhesive function, CD2 is implicated in triggering T-cell responses, with its cytoplasmic domain playing a role in signaling pathways. The protein interacts with CD48, CD58 (LFA-3), CD2AP, and PSTPIP1, contributing to a complex network of molecular interactions. Additionally, the interaction between CD2 and FCGR3A modulates NK cell activation and cytotoxicity, highlighting its diverse involvement in immune cell functions.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.571 ng/mL, corresponding to a specific activity is 1.7513×106 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.571 ng/mL, corresponding to a specific activity is 1.7513×106 units/mg.
Species

Cynomolgus

Source

HEK293

Tag

C-6*His

Accession

Q6SZ61 (K25-D209)

Gene ID

102120798  [NCBI]

Molecular Construction
N-term
CD2 (K25-D209)
Accession # Q6SZ61
6*His
C-term
Synonyms
T-cell surface antigen CD2; LFA-2; CD2; SRBC
AA Sequence

KEIRNALETWGALGQDIDLDIPSFQMSDDIDDIRWEKTSDKKKIAQFRKEKETFEEKDAYKLFKNGTLKIKHLKIHDQDSYKVSIYDTKGKNVLEKTFDLKIQERVSEPKISWTCINTTLTCEVMNGTDPELNLYQDGKHVKLSQRVITHKWTTSLSAKFKCTAGNKVSKESRMETVSCPEKGLD

Molecular Weight

Approximately 33.91 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD2 Protein, Cynomolgus (HEK293, C-His)
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HY-P75434A
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