CD229/SLAMF3 Protein, Mouse (HEK293, His)

The CD229/SLAMF3 protein is an autoligand receptor in the SLAM family that regulates immune cell activation and differentiation through homotypic or heterotypic interactions.These interactions are mediated by cytoplasmic adapters such as SH2D1A/SAP or SH2D1B/EAT-2 and are critical for coordinating innate and adaptive immune responses.CD229/SLAMF3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD229/SLAMF3 protein, expressed by HEK293 , with C-6*His labeled tag.

The CD229/SLAMF3 protein serves as a self-ligand receptor within the signaling lymphocytic activation molecule (SLAM) family, influencing immune cell activation and differentiation through homo- or heterotypic cell-cell interactions. These interactions, regulated by the presence or absence of cytoplasmic adapter proteins such as SH2D1A/SAP and/or SH2D1B/EAT-2, play a crucial role in coordinating both innate and adaptive immune responses. CD229's involvement extends to adhesion reactions between T lymphocytes and accessory cells via homophilic interaction. Additionally, it contributes to T-cell differentiation, promoting a Th17 phenotype with increased IL-17 secretion, a process dependent on the presence of SH2D1A. CD229 also plays a role in maintaining peripheral cell tolerance by acting as a negative regulator of the immune response, potentially disabling autoantibody responses and inhibiting IFN-gamma secretion by CD4(+) T-cells. Moreover, it may negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells. Furthermore, CD229 can enhance natural killer (NK) cell activation, and it interacts with SH2D1A, INPP5D, and PTPN11, with the latter interaction being blocked by SH2D1A.

Measured by its binding ability in a functional ELISA. Immobilized mouse CD229 at 5 µg/mL (100 µL/well) can bind biotinylated mouse CD229. The ED₅₀ for this effect is 1.077 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized mouse CD229 at 5 µg/mL (100 µL/well) can bind biotinylated mouse CD229. The ED₅₀ for this effect is 1.077 μg/mL.

Mouse

HEK293

C-6*His

Q4VBG4 (K48-F454)

17085  [NCBI]

Partial

KETPPTVISGMLGGSVTFSLNISKDAEIEHITWNCPPKALALVSYKKDITILDKGYNGRLKVSEDGYSLYMSNLTKSDSGSYYAQINQKNVTLTTNKEFTLHIYEKLQKPQIIVESVTPSDTDSCTFTLICTVKGTKDSVQYSWTREDTHLNTYDGSHTLRVSQSVCDPDLPYTCKAWNPVSQNSSQPVRIWQFCTGASRRKTAAGKTVVGILGEPVTLPLEFRATRATKNVVWVFNTSVISQERRGAATADSRRKPKGSEERRVRTSDQDQSLKISQLKMEDAGPYHAYVCSEASRDPSVRHFTLLVYKRLEKPSVTNSPVHMMNGICKVVLTCSVDGGGNNVTYTWMPLQNKAVMSQGKSHLNVSWESGEHLPNFTCTAHNPVSNSSSQFSSGTICSGPERNKRF

Approximately 65-75 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.