CD38 Protein, Human (Biotinylated, HEK293, His-Avi)

The CD38 protein plays a key role in cell signaling, proficiently synthesizing cyclic ADP-ribose (cADPR) as a critical second messenger for glucose-induced insulin secretion. It also promotes the synthesis of the calcium mobilizer niacin-adenine dinucleotide phosphate (NAADP+). CD38 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD38 protein, expressed by HEK293 , with N-Avi, N-6*His labeled tag.

The CD38 Protein assumes a pivotal role in cellular signaling, being a proficient synthesizer of cyclic ADP-ribose (cADPR), a recognized second messenger crucial for glucose-induced insulin secretion. Moreover, it facilitates the synthesis of the calcium mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), derived from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. Operating at both pH 5.0 and pH 7.4, CD38 Protein exhibits a preference for transforming 2'-phospho-cADPR into NAADP(+) while selectively cleaving NADP(+) to cADPR and ADPRP rather than generating NADDP(+). Notably, it also showcases cADPR hydrolase activity, highlighting its multifaceted role in the dynamic regulation of these crucial signaling molecules. The integration of these activities underscores the significance of CD38 Protein in modulating cellular responses, particularly in the context of insulin secretion and calcium mobilization.

1.Immobilized Anti-Human CD38 mAb at 2 μg/mL (100 μL/well) can bind Human CD38-His-Avi. The ED₅₀ of Human CD38-His-Avi is 13-24.54 ng/mL.
2.Loaded Biotinylated Human CD38-His on HIS1K Biosensor, can bind Anti-Human CD38 mAb-Fc with an affinity constant of 0.006 nM as determined in BLI assay.

Human

HEK293

N-Avi;N-6*His

P28907-1 (V43-I300)

952  [NCBI]

Extracellular Domain

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI

40-50 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.