CD3E-CD3G Heterodimer Protein, Mouse (HEK293, His, Flag)
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His;C-Flag
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Accession
P22646-1 (D23-D108)& P11942-1 (Q23-S116)
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Gene ID12501&12502
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Molecular Construction
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N-term
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CD3E (D23-D108)
Accession # P22646-1 -
His
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C-term
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N-term
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CD3G (Q23-S116)
Accession # P11942-1 -
Flag
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C-term
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Synonyms
CD3E; T3E; CD3 Epsilon Subunit Of T-Cell Receptor Complex; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3; T-Cell Surface Glycoprotein CD3 Epsilon Chain; CD3e Molecule; CD3-Epsilon; CD3e Antigen; CD3epsilon; CD3E Protein; CD3e Antigen, Epsilon Pol
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Predicted Molecular Mass
15.1 kDa (CD3E) & 15.2 kDa (CD3D)
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Molecular Weight
Approximately 19-22 kDa under reduced (R) conditions, 35 kDa under non reducing (N) conditions, based on SDS-PAGE, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)