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  4. CD3E-CD3G Heterodimer Protein, Mouse (HEK293, His, Flag)

CD3E-CD3G Heterodimer Protein, Mouse (HEK293, His, Flag)

Art. -Nr.: HY-P704216
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  • Technical Parameters

  • Properties

  • Beschreibung

Species

Mouse

Source

HEK293

Tag

C-His;C-Flag

Accession

P22646-1 (D23-D108)& P11942-1 (Q23-S116)

Gene ID

12501&12502

Molecular Construction
N-term
CD3E (D23-D108)
Accession # P22646-1
His
C-term
N-term
CD3G (Q23-S116)
Accession # P11942-1
Flag
C-term
Synonyms
CD3 epsilon & CD3 gamma; LCD3E & CD3G
Predicted Molecular Mass
15.1 kDa (CD3E) & 15.2 kDa (CD3D)
Molekulargewicht

Approximately 19-22 kDa,based on SDS-PAGE under reducing conditions,35 kDa,based on SDS-PAGE under non-reduced conditions,due to the glycosylation.

Glycosylation
Yes
Structure/Form
Monomer
Reinheit

≥ 90%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Versand

Room temperature in continental US; may vary elsewhere.

Dokumentation

CD3E-CD3G Heterodimer Protein, Mouse (HEK293, His, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD3E-CD3G Heterodimer Protein, Mouse (HEK293, His, Flag)

 

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Produktname:
CD3E-CD3G Heterodimer Protein, Mouse (HEK293, His, Flag)
Art. -Nr.:
HY-P704216
Menge:
MCE Japan Authorized Agent: