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  4. Integrin Associated Protein/CD47
  5. CD47 Protein, Mouse (140a.a, HEK293, His)

CD47 Protein, Mouse (140a.a, HEK293, His)

Cat. No.: HY-P72918
COA Handling Instructions

CD47 Protein, an adhesive multifunctional protein, orchestrates cell-to-cell interactions, serving as a receptor for THBS1 and modulating integrin signaling via heterotrimeric G proteins. It plays a pivotal role in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, immunoregulation, and memory formation. CD47, a receptor for SIRPA, influences dendritic cell maturation, inhibits cytokine production, and mediates cell-cell adhesion. Additionally, it modulates pulmonary EDN1 signaling, supports blood pressure in response to THBS1-induced NO signaling, and contributes to memory formation. CD47's role in wound healing, stem cell self-renewal inhibition, and its interactions with THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2, and possibly fibrinogen underscore its diverse impact on cellular processes. Existing as a monomer, CD47 exhibits intricate involvement in various cellular pathways. CD47 Protein, Mouse (140a.a, HEK293, His) is the recombinant mouse-derived CD47 protein, expressed by HEK293, with C-His labeled tag. The total length of CD47 Protein, Mouse (140a.a, HEK293, His) is 122 a.a., with molecular weight of 34-44 kDa.

For research use only. We do not sell to patients.

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Description

CD47 Protein, an adhesive multifunctional protein, orchestrates cell-to-cell interactions, serving as a receptor for THBS1 and modulating integrin signaling via heterotrimeric G proteins. It plays a pivotal role in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, immunoregulation, and memory formation. CD47, a receptor for SIRPA, influences dendritic cell maturation, inhibits cytokine production, and mediates cell-cell adhesion. Additionally, it modulates pulmonary EDN1 signaling, supports blood pressure in response to THBS1-induced NO signaling, and contributes to memory formation. CD47's role in wound healing, stem cell self-renewal inhibition, and its interactions with THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2, and possibly fibrinogen underscore its diverse impact on cellular processes. Existing as a monomer, CD47 exhibits intricate involvement in various cellular pathways. CD47 Protein, Mouse (140a.a, HEK293, His) is the recombinant mouse-derived CD47 protein, expressed by HEK293, with C-His labeled tag. The total length of CD47 Protein, Mouse (140a.a, HEK293, His) is 122 a.a., with molecular weight of 34-44 kDa.

Background

CD47 Protein, an adhesive protein, orchestrates cell-to-cell interactions and acts as a receptor for thrombospondin THBS1, concurrently modulating integrin signaling through the activation of heterotrimeric G proteins. This multifaceted protein is intricately involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. CD47 plays a pivotal role in modulating pulmonary endothelin EDN1 signaling and acts as a pressor agent supporting blood pressure in response to THBS1-induced nitrous oxide (NO) signaling. Additionally, it contributes significantly to memory formation and synaptic plasticity in the hippocampus. As a receptor for SIRPA, CD47 prevents the maturation of immature dendritic cells, inhibits cytokine production by mature dendritic cells, and mediates cell-cell adhesion through interaction with SIRPG. Furthermore, it positively modulates FAS-dependent apoptosis in T-cells and suppresses angiogenesis, potentially influencing metabolic dysregulation during normal aging. CD47's role in wound healing modulation, inhibition of stem cell self-renewal, potential involvement in membrane transport, integrin-dependent signal transduction, and prevention of premature elimination of red blood cells underscores its diverse impact on cellular processes. Existing as a monomer, CD47 interacts with THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2, and possibly fibrinogen, emphasizing its intricate involvement in a wide array of cellular pathways.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized CD47 Protein, Mouse (HEK293, His) at 10μg/mL (100μL/well) can bind mouse SIRPA-Fc and the EC50 is 0.07-0.3 μg/mL.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q61735-1 (Q19-K140)

Gene ID

16423  [NCBI]

Synonyms
Leukocyte Surface Antigen CD47; IAP; CD47; MER6
AA Sequence

MWPLAAALLLGSCCCGSAQLLFSNVNSIEFTSCNETVVIPCIVRNVEAQSTEEMFVKWKLNKSYIFIYDGNKNSTTTDQNFTSAKISVSDLINGIASLKMDKRDAMVGNYTCEVTELSREGKTVIELKNRTVSWFSPNEK

Molecular Weight

34-44 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD47 Protein, Mouse (140a.a, HEK293, His)
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HY-P72918
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