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  5. CD47 Protein, Rat (HEK293, His)

CD47 Protein, Rat (HEK293, His)

Cat. No.: HY-P75401
COA Handling Instructions

CD47 protein, acting as a receptor for THBS1 and modulating integrin signaling, facilitates cell-to-cell interactions. It plays roles in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, immunoregulation, and memory formation. CD47 interacts with SIRPA, SIRPG, FAS/CD95, UBQLN1, UBQLN2, and fibrinogen, enhancing adhesion, T-cell proliferation, and activation. It suppresses angiogenesis, regulates wound healing and stem cell self-renewal, and prevents premature elimination of red blood cells. CD47 influences pulmonary endothelin signaling, NO signaling, and blood pressure regulation. It may also contribute to metabolic dysregulation during aging. CD47 Protein, Rat (HEK293, His) is the recombinant rat-derived CD47 protein, expressed by HEK293, with C-His labeled tag. The total length of CD47 Protein, Rat (HEK293, His) is 122 a.a., with molecular weight of ~23-40 kDa.

For research use only. We do not sell to patients.

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Description

CD47 protein, acting as a receptor for THBS1 and modulating integrin signaling, facilitates cell-to-cell interactions. It plays roles in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, immunoregulation, and memory formation. CD47 interacts with SIRPA, SIRPG, FAS/CD95, UBQLN1, UBQLN2, and fibrinogen, enhancing adhesion, T-cell proliferation, and activation. It suppresses angiogenesis, regulates wound healing and stem cell self-renewal, and prevents premature elimination of red blood cells. CD47 influences pulmonary endothelin signaling, NO signaling, and blood pressure regulation. It may also contribute to metabolic dysregulation during aging. CD47 Protein, Rat (HEK293, His) is the recombinant rat-derived CD47 protein, expressed by HEK293, with C-His labeled tag. The total length of CD47 Protein, Rat (HEK293, His) is 122 a.a., with molecular weight of ~23-40 kDa.

Background

The CD47 protein is an adhesive protein that facilitates cell-to-cell interactions. It acts as a receptor for thrombospondin THBS1 and modulates integrin signaling through the activation of heterotrimeric G proteins. CD47 is involved in various biological processes, including signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. It also plays a role in modulating pulmonary endothelin EDN1 signaling and nitrous oxide (NO) signaling, contributing to blood pressure regulation. Additionally, CD47 is important for memory formation and synaptic plasticity in the hippocampus. It serves as a receptor for SIRPA, preventing maturation of immature dendritic cells and inhibiting cytokine production by mature dendritic cells. Interaction with SIRPG enhances cell-cell adhesion, T-cell proliferation, and T-cell activation. CD47 positively modulates FAS-dependent apoptosis in T-cells and suppresses angiogenesis. It may also be involved in metabolic dysregulation during normal aging and regulate wound healing and stem cell self-renewal. CD47 may play a role in membrane transport, integrin-dependent signal transduction, and prevention of premature elimination of red blood cells. It interacts with THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2, and fibrinogen.

Biological Activity

Immobilized Mouse SIRP alpha, Mouse at 10 μg/mL (100 μL/well) can bind Rat CD47 with a linear range of 117.2 ng/mL.

  • Immobilized Mouse SIRP alpha, Mouse at 10 μg/mL (100 μL/well) can bind Rat CD47with a linear range of 117.2 ng/mL .
Species

Rat

Source

HEK293

Tag

C-His

Accession

P97829 (Q19-K140)

Gene ID

29364  [NCBI]

Molecular Construction
N-term
CD47 (Q19-K140)
Accession # P97829
His
C-term
Synonyms
Leukocyte Surface Antigen CD47; IAP; CD47; MER6
AA Sequence

QLLLSKVKSVEFTSCNDTVVIPCKVLNVEAQSTDEMFVKWKLNKSYIFIYDGNKNSTTREQNFTSAKISVSDLLKGIASLTMDTHEAVVGNYTCEVTELSREGKTVIELKNRPVSWFSTNEK

Molecular Weight

The protein migrates as an approximately 23-40 kDa band under reducing SDS-PAGE due to glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD47 Protein, Rat (HEK293, His)
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HY-P75401
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