CD98 Protein, Human (HEK293, hFc)

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CD98 Protein, Human (HEK293, hFc) is the recombinant human-derived CD98 protein, expressed by HEK293, with N-hFc tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

CD98 Protein, Human (HEK293, hFc) is the recombinant human-derived CD98 protein, expressed by HEK293, with N-hFc tag.

Background

CD98, a multifunctional protein, is predicted to possess amino acid transmembrane transporter activity and double-stranded RNA binding activity. Its involvement in carboxylic acid transport and viral entry into host cells, as well as its role upstream of response to exogenous dsRNA, highlights its diverse functions. CD98 is strategically located in the apical pole of neurons and is actively engaged in synaptic processes. Its expression spans various structures, including the alimentary system, brain, genitourinary system, hemolymphoid system gland, and spinal cord. The ubiquity of CD98 expression is evident in numerous tissues, such as the kidney, genital fat pad, and 28 other tissues, underscoring its importance in various physiological contexts. Elucidating the intricate roles of CD98 in transport, cellular entry, and synaptic function is essential for comprehending its contributions to cellular processes and potential implications in health and disease.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized Human CD98, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate can bind Biotinylated CD98 Antibody, hFc Tag. Test result was comparable to standard batch.
2.Immobilized Human CD98, hFc Tag at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Biotinylated CD98 Antibody, hFc Tag with the ED50 of 17.7 ng/ml determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-hFc
  • Accession
  • Gene ID
  • Protein Length

    Partial

  • Synonyms

    SLC3A2; Solute Carrier Family 3 (Activators Of Dibasic And Neutral Amino Acid Transport), Member 2; Prev. MDU1; Solute Carrier Family 3 (Amino Acid Transporter Heavy Chain), Member 2; 4F2 Cell-Surface Antigen Heavy Chain; Monoclonal Antibody 44D7; Lymphoc

  • AA Sequence

    RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

  • Predicted Molecular Mass

    72.4 kDa

  • Molecular Weight

    Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.;≥ 95%, as determined by HPLC.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<0.01 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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