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CDH16 Protein, Human (HEK293, C-His)

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Based on 1 Customer Validation

CDH16, a calcium-dependent cell adhesion protein, belongs to the cadherin family. It exhibits homophilic interactions, potentially contributing to cell-type sorting and regulating cell-cell adhesions for cellular organization. CDH16 Protein, Human (HEK293, C-His) is the recombinant human-derived CDH16 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CDH16 Protein, Human (HEK293, C-His)
  • Experimental Validation Results for CDH16 Protein, Human (HEK293, C-His)
  • CDH16 Protein, Human (HEK293, C-His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CDH16, a calcium-dependent cell adhesion protein, belongs to the cadherin family. It exhibits homophilic interactions, potentially contributing to cell-type sorting and regulating cell-cell adhesions for cellular organization. CDH16 Protein, Human (HEK293, C-His) is the recombinant human-derived CDH16 protein, expressed by HEK293 , with C-His labeled tag.

Background

CDH16, a member of the cadherin family, is a calcium-dependent cell adhesion protein. It exhibits a preference for homophilic interactions, engaging with itself in connecting cells. This characteristic suggests that CDH16, like other cadherins, may play a role in the sorting of heterogeneous cell types, contributing to the regulation of cell-cell adhesions and maintaining cellular organization.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized CDH16 at 0.5 μg/mL (100μL/well) can bind CDH16 antibody, the ED50 for this effect is 5.963 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized CDH16 at 0.5 μg/mL (100μL/well) can bind CDH16 antibody, the ED50 for this effect is 5.963 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    O75309/NP_004053.1 (K19-A786)

  • Gene ID
    1014
  • Molecular Construction
    • N-term
    • CDH16 (K19-A786)
      Accession # O75309/NP_004053.1
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CDH16; Cadherin 16, KSP-Cadherin; Cadherin 16; KSP-Cadherin; Kidney-Specific Cadherin; Ksp-Cadherin; Cadherin-16

  • AA Sequence

    KAQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSPPGTEVTRLSAEDADAPGSPNSHVVYQLLSPEPEDGVEGRAFQVDPTSGSVTLGVLPLRAGQNILLLVLAMDLAGAEGGFSSTCEVEVAVTDINDHAPEFITSQIGPISLPEDVEPGTLVAMLTAIDADLEPAFRLMDFAIERGDTEGTFGLDWEPDSGHVRLRLCKNLSYEAAPSHEVVVVVQSVAKLVGPGPGPGATATVTVLVERVMPPPKLDQESYEASVPISAPAGSFLLTIQPSDPISRTLRFSLVNDSEGWLCIEKFSGEVHTAQSLQGAQPGDTYTVLVEAQDTDEPRLSASAPLVIHFLKAPPAPALTLAPVPSQYLCTPRQDHGLIVSGPSKDPDLASGHGPYSFTLGPNPTVQRDWRLQTLNGSHAYLTLALHWVEPREHIIPVVVSHNAQMWQLLVRVIVCRCNVEGQCMRKVGRMKGMPTKLSA

  • Predicted Molecular Mass

    84.6 kDa

  • Molecular Weight

    Approximately 90-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 100 mM NaCl, 1mM EDTA, 200 mM Arginine pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized CDH16 at 0.5 μg/mL (100μL/well) can bind CDH16 antibody, the ED50 for this effect is 5.963 ng/mL.

  • CDH16 Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for CDH16 Protein, Human (HEK293, C-His)
  • Experimental Validation Results for CDH16 Protein, Human (HEK293, C-His)
  • CDH16 Protein, Human (HEK293, C-His) Product Image