CENPE Protein, Human
Based on 1 Customer Validation
CENPE Protein, Human is the recombinant human-derived CENPE, expressed by E. coli , with tag Free labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
CENPE Protein, Human is the recombinant human-derived CENPE, expressed by E. coli , with tag Free labeled tag.
CENPE is a microtubule plus-end-directed kinetochore motor protein that plays pivotal roles in chromosome congression, microtubule-kinetochore conjugation, and spindle assembly checkpoint (SAC) activation. It drives chromosome congression (metaphase plate formation) by mediating lateral sliding of polar chromosomes along spindle microtubules toward the equator and facilitating kinetochore-microtubule attachment stabilization. Dettyrosinated microtubule tracks preferentially transport pole-proximal chromosomes. As a processive bi-directional tracker of dynamic microtubule tips, CENPE persistently strengthens kinetochore-microtubule linkages post-congression. It suppresses congression in NDC80-depleted cells and promotes it only when microtubules are stabilized. CENPE is essential for stable kinetochore-microtubule attachments, requiring its dependent recruitment of BUB1B, MAD1, and MAD2 to kinetochores. Through interaction with BUB1B, it activates SAC kinase activity to prevent aneuploidy from single chromosome loss (by similar).
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q02224 (M1-S339)
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Gene ID1062
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Protein Length
Partial
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Synonyms
CENPE; Centromere Protein E, 312kDa; Centromere Protein E; Kinesin-7; PPP1R61; CENP-E; KIF10; Centromere Autoantigen E (312kD); Protein Phosphatase 1, Regulatory Subunit 61; Centromere Protein E (312kD); Centromere-Associated Protein E; Kinesin Family Mem
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AA Sequence
MAEEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGSKSFNFDRVFHGNETTKNVYEEIAAPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSEDHLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNRNVYVADLTEEVVYTSEMALKWITKGEKSRHYGETKMNQRSSRSHTIFRMILESREKGEPSNCEGSVKVSHLNLVDLAGSERAAQTGAAGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPVSFDETLTALQFASTAKYMKNTPYVNEVS
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Predicted Molecular Mass
38.2 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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