CHAC1 Protein, Human (His, Strep)
The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human (His, Strep) is the recombinant human-derived CHAC1 protein, expressed by E. coli , with C-Strep, N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human (His, Strep) is the recombinant human-derived CHAC1 protein, expressed by E. coli , with C-Strep, N-6*His labeled tag.
Background
CHAC1, a key player in cellular processes, catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide, exhibiting specificity for glutathione over other gamma-glutamyl peptides. Its enzymatic action on glutathione is crucial as glutathione depletion is a significant factor in apoptosis initiation and execution. CHAC1 further serves as a pro-apoptotic component within the unfolded protein response pathway, mediating the effects of the ATF4-ATF3-DDIT3/CHOP cascade. Additionally, CHAC1 functions as a negative regulator of the Notch signaling pathway, impacting embryonic neurogenesis by inhibiting Notch cleavage through furin. This inhibition maintains Notch in an immature, inactive form, thereby promoting neurogenesis in embryos.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His;C-Strep
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Accession
Q9BUX1 (K2-V222)
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Gene ID79094
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Molecular Construction
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N-term
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Strep-6*His
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CHAC1 (K2-V222)
Accession # Q9BUX1 -
C-term
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Protein Length
Full Length
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Synonyms
CHAC1; Botch; ChaC Glutathione Specific Gamma-Glutamylcyclotransferase 1; ChaC, Cation Transport Regulator Homolog 1 (E. Coli); Glutathione-Specific Gamma-Glutamylcyclotransferase 1; ChaC, Cation Transport Regulator Homolog 1; Gamma-GCT Acting On Glutathi
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AA Sequence
KQESAAPNTPPTSQSPTPSAQFPRNDGDPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLNVREAVLGGYDTKEVTFYPQDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLAAIVDAVGTMLPCFCPTEQALALV
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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