CHT Protein, Human (HEK293, Strep, His)

CHT Protein, Human (HEK293, Strep, His) is the recombinant human-derived CHT, expressed by HEK293 , with Strep, His labeled tag.

CHT is a high-affinity Na(+)-coupled choline transmembrane symporter that functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current are Cl(-)-dependent, where Cl(-) likely acts as a regulatory ion rather than a cotransported ion. It plays a critical role in acetylcholine (ACh) synthesis by transporting choline from the synaptic cleft into presynaptic nerve terminals. SLC5A7/CHT1-mediated high-affinity choline transport in cholinergic neurons is the rate-limiting step for ACh production, facilitating subsequent action potential transmission. CHT is predominantly localized in presynaptic terminal intracellular organelles and translocates to the plasma membrane in its active form in response to neuronal activity.

Human

HEK293

Strep;His

Q9GZV3 (M1-Q580)

60482

Full Length

MAFHVEGLIAIIVFYLLILLVGIWAAWRTKNSGSAEERSEAIIVGGRDIGLLVGGFTMTATWVGGGYINGTAEAVYVPGYGLAWAQAPIGYSLSLILGGLFFAKPMRSKGYVTMLDPFQQIYGKRMGGLLFIPALMGEMFWAAAIFSALGATISVIIDVDMHISVIISALIATLYTLVGGLYSVAYTDVVQLFCIFVGLWISVPFALSHPAVADIGFTAVHAKYQKPWLGTVDSSEVYSWLDSFLLLMLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFGCLVMAIPAILIGAIGASTDWNQTAYGLPDPKTTEEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADSSILSASSMFARNIYQLSFRQNASDKEIVWVMRITVFVFGASATAMALLTKTVYGLWYLSSDLVYIVIFPQLLCVLFVKGTNTYGAVAGYVSGLFLRITGGEPYLYLQPLIFYPGYYPDDNGIYNQKFPFKTLAMVTSFLTNICISYLAKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVKNENIKLDELALVKPRQSMTLSSTFTNKEAFLDVDSSPEGSGTEDNLQ

Approximately 50 kDa, based on SDS-PAGE under reducing conditions.

≥ 80%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.004% (w/v) LMNG.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.