CHT Protein, Human (HEK293, Strep, His)
Based on 1 Customer Validation
CHT Protein, Human (HEK293, Strep, His) is the recombinant human-derived CHT, expressed by HEK293 , with Strep, His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CHT Protein, Human (HEK293, Strep, His) is the recombinant human-derived CHT, expressed by HEK293 , with Strep, His labeled tag.
Background
CHT is a high-affinity Na(+)-coupled choline transmembrane symporter that functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current are Cl(-)-dependent, where Cl(-) likely acts as a regulatory ion rather than a cotransported ion. It plays a critical role in acetylcholine (ACh) synthesis by transporting choline from the synaptic cleft into presynaptic nerve terminals. SLC5A7/CHT1-mediated high-affinity choline transport in cholinergic neurons is the rate-limiting step for ACh production, facilitating subsequent action potential transmission. CHT is predominantly localized in presynaptic terminal intracellular organelles and translocates to the plasma membrane in its active form in response to neuronal activity.
Technical Parameters
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Species Human
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Source HEK293
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Tag Strep;His
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Accession
Q9GZV3 (M1-Q580)
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Gene ID60482
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Protein Length
Full Length
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Synonyms
SLC5A7; Solute Carrier Family 5 (Choline Transporter), Member 7; Solute Carrier Family 5 Member 7; Motor Neuronopathy, Distal Hereditary, Type VIIA; CHT1; Hemicholinium-3-Sensitive Choline Transporter; High Affinity Choline Transporter 1; SLC5A7 Protein;
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AA Sequence
MAFHVEGLIAIIVFYLLILLVGIWAAWRTKNSGSAEERSEAIIVGGRDIGLLVGGFTMTATWVGGGYINGTAEAVYVPGYGLAWAQAPIGYSLSLILGGLFFAKPMRSKGYVTMLDPFQQIYGKRMGGLLFIPALMGEMFWAAAIFSALGATISVIIDVDMHISVIISALIATLYTLVGGLYSVAYTDVVQLFCIFVGLWISVPFALSHPAVADIGFTAVHAKYQKPWLGTVDSSEVYSWLDSFLLLMLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFGCLVMAIPAILIGAIGASTDWNQTAYGLPDPKTTEEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADSSILSASSMFARNIYQLSFRQNASDKEIVWVMRITVFVFGASATAMALLTKTVYGLWYLSSDLVYIVIFPQLLCVLFVKGTNTYGAVAGYVSGLFLRITGGEPYLYLQPLIFYPGYYPDDNGIYNQKFPFKTLAMVTSFLTNICISYLAKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVKNENIKLDELALVKPRQSMTLSSTFTNKEAFLDVDSSPEGSGTEDNLQ
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Predicted Molecular Mass
67.6 kDa
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM HEPES, pH 7.5, 150 mM NaCl, 0.004% (w/v) LMNG.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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