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CKAP4 Protein, Human (His)

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CKAP1/TBCB Protein plays a crucial role in cellular functions, anchoring the endoplasmic reticulum to microtubules for structural organization. Moreover, it serves as a high-affinity epithelial cell surface receptor for APF/antiproliferative factor, transmitting antiproliferative signals. This dual functionality underscores CKAP1/TBCB's significance in both intracellular structural dynamics and cellular signaling processes. CKAP4 Protein, Human (His) is the recombinant human-derived CKAP4 protein, expressed by E. coli, with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

CKAP1/TBCB Protein plays a crucial role in cellular functions, anchoring the endoplasmic reticulum to microtubules for structural organization. Moreover, it serves as a high-affinity epithelial cell surface receptor for APF/antiproliferative factor, transmitting antiproliferative signals. This dual functionality underscores CKAP1/TBCB's significance in both intracellular structural dynamics and cellular signaling processes. CKAP4 Protein, Human (His) is the recombinant human-derived CKAP4 protein, expressed by E. coli, with N-His labeled tag.

Background

CKAP4 is a protein with multiple functions. Its core role includes mediating the anchoring of the endoplasmic reticulum and microtubules, thereby maintaining the stability of the intracellular structure. At the same time, as a high-affinity receptor on the surface of epithelial cells, it can bind to the FZD8-related low molecular weight sialic acid glycopeptide APF (anti-proliferation factor) and mediate the anti-proliferation signal transduction of APF in cells, playing an important role in maintaining cell structure and regulating proliferation.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q07065 (H128-V602)

  • Gene ID
    10970  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • CKAP4 (H128-V602)
      Accession # Q07065
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CKAP4; 63-KDa Cytoskeleton-Linking Membrane Protein; Cytoskeleton Associated Protein 4; Transmembrane Protein (63kD), Endoplasmic Reticulum/Golgi Intermediate Compartment; P63; Type-II Transmembrane Protein P63; Cytoskeleton-Associated Protein 4; 63 KDa M

  • AA Sequence

    HHVLEEVQQVRRSHQDFSRQREELGQGLQGVEQKVQSLQATFGTFESILRSSQHKQDLTEKAVKQGESEVSRISEVLQKLQNEILKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV

  • Predicted Molecular Mass

    53.7 kDa

  • Molecular Weight

    Approximately 54 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, 200 mM NaCl, 10% glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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