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  3. Transferases (EC 2)
  4. CKMT1A Protein, Human (sf9, His)

CKMT1A Protein, Human (sf9, His)

Cat. No.: HY-P76264
COA Handling Instructions

CKMT1A is a creatine kinase isoenzyme that plays a key role in the reversible transfer of phosphate between ATP and creatine phosphate. The function of this enzyme is critical for tissues with dynamic energy needs, such as skeletal muscle, heart, brain, and sperm. CKMT1A Protein, Human (sf9, His) is the recombinant human-derived CKMT1A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of CKMT1A Protein, Human (sf9, His) is 378 a.a., with molecular weight of ~43 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

CKMT1A is a creatine kinase isoenzyme that plays a key role in the reversible transfer of phosphate between ATP and creatine phosphate. The function of this enzyme is critical for tissues with dynamic energy needs, such as skeletal muscle, heart, brain, and sperm. CKMT1A Protein, Human (sf9, His) is the recombinant human-derived CKMT1A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of CKMT1A Protein, Human (sf9, His) is 378 a.a., with molecular weight of ~43 KDa.

Background

The CKMT1A protein, a creatine kinase isoenzyme, is instrumental in reversibly catalyzing the transfer of phosphate between ATP and various phosphogens, notably creatine phosphate. This enzymatic activity holds significant importance in tissues with substantial and fluctuating energy demands, including skeletal muscle, heart, brain, and spermatozoa. Creatine kinase isoenzymes, including CKMT1A, play a central role in energy transduction, facilitating the efficient utilization and storage of energy in response to dynamic metabolic requirements in these tissues.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

P12532 (A40-H417)

Gene ID
Molecular Construction
N-term
His
CKMT1A (A40-H417)
Accession # P12532
C-term
Synonyms
Creatine kinase U-type, mitochondrial; Mia-CK; U-MtCK; CKMT; CKMT1B
Molecular Weight

Approximately 43 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% gly.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

CKMT1A Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CKMT1A Protein, Human (sf9, His)
Cat. No.:
HY-P76264
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