CLEC1B/CLEC-2 Protein, Human (HEK293, His)

CLEC1B/CLEC-2 protein is a C-type lectin-like receptor that acts as a platelet receptor and snake venom protein receptor for PDPN in microbial infections. Upon activation, it initiates a signaling cascade involving SRC and SYK kinases, leading to PLCG2 activation. CLEC1B/CLEC-2 Protein, Human (HEK293, His) is the recombinant human-derived CLEC1B/CLEC-2, expressed by HEK293, with N-10*His labeled tag.

CLEC1B/CLEC-2 protein serves as a C-type lectin-like receptor with a notable function as a platelet receptor for the lymphatic endothelial marker, PDPN. Upon ligand activation, it initiates signaling cascades involving the sequential activation of SRC and SYK tyrosine kinases, ultimately leading to the activation of PLCG2. In the context of microbial infection, CLEC1B/CLEC-2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding induces tyrosine phosphorylation, facilitating the binding of spleen tyrosine kinase (SYK) and initiating downstream tyrosine phosphorylation events, along with the activation of PLCG2. This multifaceted role highlights the involvement of CLEC1B/CLEC-2 in platelet function and immune responses, particularly in the context of interactions with PDPN and rhodocytin.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Podoplanin can bind Recombinant Human CLEC-2/CLEC1B. The kD is 3.498 nM.

Human

HEK293

N-10*His

Q9P126-1 (Q58-P229)

51266

Extracellular Domain

QRNYLQGENENRTGTLQQLAKRFCQYVVKQSELKGTFKGHKCSPCDTNWRYYGDSCYGFFRHNLTWEESKQYCTDMNATLLKIDNRNIVEYIKARTHLIRWVGLSRQKSNEVWKWEDGSVISENMFEFLEDGKGNMNCAYFHNGKMHPTFCENKHYLMCERKAGMTKVDQLP

Approximately 30-38 kDa due to the glycosylation

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.