CNPY2 Protein, Human (HEK293, His)

CNPY2 Protein, a positive regulator of neurite outgrowth, stabilizes the myosin regulatory light chain (MRLC) by preventing MIR-mediated ubiquitination and proteasomal degradation. The interaction with MYLIP/MIR highlights CNPY2's crucial role in facilitating MRLC stability and promoting neurite outgrowth. CNPY2 Protein, Human (HEK293, His) is the recombinant human-derived CNPY2 protein, expressed by HEK293 , with C-His labeled tag. The total length of CNPY2 Protein, Human (HEK293, His) is 178 a.a., with molecular weight of ~19 kDa.

CNPY2, a positive regulator of neurite outgrowth, exerts its function by stabilizing the myosin regulatory light chain (MRLC). It plays a crucial role in preventing MIR-mediated ubiquitination of MRLC, thereby averting its subsequent proteasomal degradation. The interaction between CNPY2 and MYLIP/MIR underscores its regulatory influence on MRLC stability, emphasizing its significance in facilitating neurite outgrowth.

Human

HEK293

C-6*His

Q9Y2B0-1 (R21-S178)

10330  [NCBI]

RRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHIS

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.