1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Neurotrophic Factors
  4. Ciliary Neurotrophic Factor Receptor (CNTFR)
  5. CNTFR alpha Protein, Human (sf9, His)

CNTFR alpha Protein, Human (sf9, His)

Cat. No.: HY-P76275
Handling Instructions

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/mL (100 μL/well) can bind biotinylated human CNTF with a linear range of 1.28-160 ng/mL.
Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P26992 (M1-P346)

Gene ID
Synonyms
Ciliary neurotrophic factor receptor subunit alpha; CNTFR-alpha
Molecular Weight

45-48 kDa.

Purity

Greater than 98 % as determined by SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CNTFR alpha Protein, Human (sf9, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CNTFR alpha Protein, Human (sf9, His)
Cat. No.:
HY-P76275
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