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  5. Coagulation Factor X/F10 Protein, Mouse (HEK293, His)

Coagulation Factor X/F10 Protein, Mouse (HEK293, His)

Cat. No.: HY-P70215
COA Handling Instructions

Coagulation Factor X/F10 Protein, a crucial vitamin K-dependent glycoprotein, is central to blood clotting. It produces Factor Xa, essential for converting prothrombin to thrombin, a process facilitated by factor Va, calcium, and phospholipid in the intricate cascade of blood coagulation. Coagulation Factor X/F10 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Coagulation Factor X/F10 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Coagulation Factor X/F10 Protein, Mouse (HEK293, His) is 461 a.a., with molecular weight of ~55.0 kDa.

For research use only. We do not sell to patients.

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Description

Coagulation Factor X/F10 Protein, a crucial vitamin K-dependent glycoprotein, is central to blood clotting. It produces Factor Xa, essential for converting prothrombin to thrombin, a process facilitated by factor Va, calcium, and phospholipid in the intricate cascade of blood coagulation. Coagulation Factor X/F10 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Coagulation Factor X/F10 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Coagulation Factor X/F10 Protein, Mouse (HEK293, His) is 461 a.a., with molecular weight of ~55.0 kDa.
Background

Coagulation Factor X/F10 Protein, a vitamin K-dependent glycoprotein, plays a pivotal role in blood clotting. Factor Xa, produced by this protein, is instrumental in converting prothrombin to thrombin. This conversion occurs in the presence of factor Va, calcium, and phospholipid, contributing to the intricate process of blood coagulation.
Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Species

Mouse
Source

HEK293
Tag

C-6*His
Accession

O88947 (G21-N481)
Gene ID

14058  [NCBI]
Molecular Construction
N-term
F10 (G21-N481)
Accession # O88947
6*His
C-term
Synonyms
rMuCoagulation factor X/FX, His; F10; Coagulation factor X; Stuart factor
AA Sequence

GKGVFINRERANNVLARTRRANSFFEEFKKGNLERECMEEICSYEEVREIFEDDEKTKEYWTKYKDGDQCESSPCQNQGACRDGIGGYTCTCSEGFEGKNCELFVRKLCRLDNGDCDQFCREEQNSVVCSCASGYFLGNDGKSCISTAPFPCGKITTGRRKRSVALNTSDSELDLEDALLDEDFLSPTENPIELLNLNETQPERSSDDLVRIVGGRECKDGECPWQALLINEDNEGFCGGTILNEFYILTAAHCLHQARRFKVRVGDRNTEKEEGNEMVHEVDVVIKHNKFQRDTYDYDIAVLRLKTPITFRMNVAPACLPQKDWAESTLMTQKTGIVSGFGRTHEKGRQSNILKMLEVPYVDRNTCKLSTSFSITQNMFCAGYEAKLEDACQGDSGGPHVTRFKNTYYVTGIVSWGEGCARKGKYGIYTKVTTFLKWIDRSMKARVGPTAETPRTAGPPN

Molecular Weight

50-60 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder
Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 1 mM CaCl2, pH 7.5.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

Coagulation Factor X/F10 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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