COL4A2 Protein, Human (His)
Based on 1 Customer Validation
COL4A2 protein is a key type IV collagen element that forms the main structural component of the glomerular basement membrane (GBM), forming a unique "chicken wire" network structure with laminin, proteoglycans and nestin/nesidin. . Canstatin is a cleavage product of the NC1 domain of collagen α 2(IV) and has potent anti-angiogenic and anti-tumor activities. COL4A2 Protein, Human (His) is the recombinant human-derived COL4A2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
COL4A2 protein is a key type IV collagen element that forms the main structural component of the glomerular basement membrane (GBM), forming a unique "chicken wire" network structure with laminin, proteoglycans and nestin/nesidin. . Canstatin is a cleavage product of the NC1 domain of collagen α 2(IV) and has potent anti-angiogenic and anti-tumor activities. COL4A2 Protein, Human (His) is the recombinant human-derived COL4A2 protein, expressed by E. coli , with N-6*His labeled tag.
COL4A2 protein, a fundamental element of Type IV collagen, plays a central role as the major structural component in glomerular basement membranes (GBM), forming a characteristic 'chicken-wire' meshwork in conjunction with laminins, proteoglycans, and entactin/nidogen. Within this context, Canstatin, a cleavage product derived from the collagen alpha 2(IV) NC1 domain, exhibits notable anti-angiogenic and anti-tumor cell activities. Canstatin interferes with the proliferation and migration of endothelial cells, diminishes mitochondrial membrane potential, and induces apoptosis. Its mechanism includes the specific induction of Fas-dependent apoptosis, along with the activation of procaspase-8 and -9 activities. Notably, Canstatin acts as a ligand for alphavbeta3 and alphavbeta5 integrins, underscoring its multifaceted role in modulating cellular responses within the intricate context of Type IV collagen-rich environments like GBMs.
Measured by its ability to inhibit the proliferation of HUVEC cells. The inhibition rate was 69.6% When human COL4A2 is 10 μg/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P08572 (V1493-L1712)
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Molecular Construction
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N-term
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6*His
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COL4A2 (V1493-L1712)
Accession # P08572 -
C-term
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Protein Length
Partial
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Synonyms
COL4A2; Collagen Type IV Alpha 2; Collagen Type IV Alpha 2 Chain; BSVD2A; Collagen Alpha-2(IV) Chain; BSVD2B; DKFZp686I14213; POREN2; Canstatin; BSVD2; FLJ22259; ICH
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AA Sequence
VKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL
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Predicted Molecular Mass
25.1 kDa
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Molecular Weight
Approximately 26 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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