COL4A2 Protein, Human (His)

COL4A2 protein is a key type IV collagen element that forms the main structural component of the glomerular basement membrane (GBM), forming a unique "chicken wire" network structure with laminin, proteoglycans and nestin/nesidin. . Canstatin is a cleavage product of the NC1 domain of collagen α 2(IV) and has potent anti-angiogenic and anti-tumor activities. COL4A2 Protein, Human (His) is the recombinant human-derived COL4A2 protein, expressed by E. coli , with N-6*His labeled tag.

COL4A2 protein, a fundamental element of Type IV collagen, plays a central role as the major structural component in glomerular basement membranes (GBM), forming a characteristic 'chicken-wire' meshwork in conjunction with laminins, proteoglycans, and entactin/nidogen. Within this context, Canstatin, a cleavage product derived from the collagen alpha 2(IV) NC1 domain, exhibits notable anti-angiogenic and anti-tumor cell activities. Canstatin interferes with the proliferation and migration of endothelial cells, diminishes mitochondrial membrane potential, and induces apoptosis. Its mechanism includes the specific induction of Fas-dependent apoptosis, along with the activation of procaspase-8 and -9 activities. Notably, Canstatin acts as a ligand for alphavbeta3 and alphavbeta5 integrins, underscoring its multifaceted role in modulating cellular responses within the intricate context of Type IV collagen-rich environments like GBMs.

Measured by its ability to inhibit the proliferation of HUVEC cells. The inhibition rate was 69.6% When human COL4A2 is 10 μg/mL.

Human

E. coli

N-6*His

P08572 (V1493-L1712)

1284  [NCBI]

Partial

VKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL

Approximately 26 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.